The size distribution of chromatin fragments released by micrococcal nuclease digestion of liver chromatin at various ionic strengths was examined. Below 20 mM ionic strength, gradient profiles with a peak centered at 6 nucleosomes are generated, whereas between 20 and 50 mM the peak is always centered on 12 nucleosomes, and above 50 mM ionic strength the 30-nm fiber becomes less accessible to ...

The thermal denaturation of ribonuclease A has been studied by differential scanning calorimetry in the presence of 4-chlorobutan-1-ol. The thermal transitions were observed to be reversible at pH 5.5 in the presence of low concentration (up to 50 mM) of the alcohol, irreversible in the intermediate (50 mM < c < mM) and again reversible in the presence of 250 mM and higher concentrations of 4-c...

Pulmonary hypertension has been associated with poor outcome after liver transplantation. We assessed the diagnostic accuracy of Doppler echocardiography in detecting significant pulmonary hypertension. Seventy-four potential liver transplant candidates underwent Doppler echocardiography in which the systolic right ventricular pressure (RVsys) was used to estimate the systolic pulmonary artery ...

Abstract Balanced levels of macronutrients and sucrose may ensure the success micropropagation endangered ones. This study aimed to evaluate effect salts in culture medium on vitro Lomatozona artemisiifolia, as well determine influence light emitting diode (LED) shoot proliferation rooting species. Nodal segments were used different macronutrient concentrations MS (25, 50 100%), (0.0 mM; 0.34 0...

BACKGROUND Vacuum-assisted closure (VAC) is a widely used therapy in patients with poststernotomy mediastinitis. The aim of this study was to evaluate sternal stability during VAC application at seven negative pressures (-50 to -200 mm Hg) in a porcine wound model. METHODS Six pigs underwent median sternotomy and 2 steel wires were fixed at each sternal side and connected to a traction device...

Background: Trehalose, a non-permeable disaccharide, has a protective role related both to osmotic effect and specific interactions with membrane phospholipids, rendering hypertonic media, causing cellular osmotic dehydration before freezing, and then decreasing the amount of cell injury by ice crystallization. Within this study, an attempt was made to evaluation of two levels of 50 and 100 mM ...

Rainfall data of 17 districts in Chhattisgarh state has been used to determine the quantity rainfall at different probability levels through incomplete gamma distribution. Bastar highest total annual rainfall, with 1090.0 mm, 1247.5 and 1440.0 respectively, 90 percent, 75 50 percent likelihood levels. Sukma district 1696.6 mm 1955.3 25% 10% levels, respectively. At Bilaspur lowest 300.8 542.8 K...

Two cathepsin B inhibitors were isolated from the culture supernatant of a marine Pseudomonas sp. PB01 (GenBank Accession No. EU126129). Their structures were elucidated by spectroscopic analyses as dibutyl phthalate and di-(2-ethylhexyl) phthalate. Both dibutyl phthalate and di-(2-ethylhexyl) phthalate showed dose-dependent cathepsin B inhibitions with IC(50) of 0.42 and 0.38 mM, respectively....