FokI is a type IIs restriction endonuclease which recognizes an asymmetric DNA sequence and cleaves DNA a short distance away from the sequence. The enzyme is bipartite in nature with its DNA recognition and cleavage functions located on distinct domains. We report here cocrystals of the complete FokI enzyme (579 amino acids) bound to a 20-bp DNA fragment containing its recognition sequence. Th...

Questions regarding the digestive fate of DNA and protein from transgenic grain have been raised in regard to human consumption and trade of animal products (e.g., meat, milk, and eggs) from farm animals fed transgenic crops. Using highly sensitive, fully characterized analytical methods, fragments of transgenic and endogenous plant DNA, as well as transgenic protein, were not detected in chick...

calpastatin (cast) is a specific inhibiter of calpains, playing a role in meat tenderization and myogenesis. in the present study the polymorphism of the cast gene ofkhalkhali goat in azerbaijan province in iran was investigated by polymerase chain reaction and restriction fragment length polymorphism technique (pcr-rflp). genomic dna was extracted from whole blood samples collected from 200 kh...

viral symptoms indicative of iris yellow spot virus (iysv) were observed on onion in several fields near chenaran in khorasan razavi province. mechanical inoculation of herbaceous hosts with onion sap extracts from symptomatic plants showed similar symptoms to those described for iysv. the mechanically transmitted virus reacted only with antisera specific to iysv in das-elisa but not with antis...

Validated protocols for DNA purification and PCR amplification are reported for detection of Phytophthora cactorum in diseased strawberry plants. To remove PCR inhibitors, necrotic strawberry tissues were soaked in 5% alconox solution for >12 h before DNA extraction, and the extracted genomic DNA was embedded in an agarose gel chamber and subjected to electrophoresis. The purified DNA was ampli...

bovine theileriosis is important disease in tropical and subtropical areas with great economic losses in livestock husbandry in iran. the aim of study was to assess the prevalence of theileria annulata infection in cattle and hyalomma species of kermanshah province, iran. a number of 138 blood samples were randomly taken from examined cattle. the genomic dna was extracted and pcr was performed ...

Division of Experimental Therapy, The Netherlands Cancer Institute Antonin van Leeuwenhoek Huis, 1066 CX Amsterdam, The Netherlands The search for sequence variations in genomic DNA becomes increasingly important in the study of inherited disease genes as well as of genes that play a role in the development of cancer. A wide variety of different methods to detect DNA sequence variations has bee...

background: haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.   methods: the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundre...

An EcoRI fragment (1.2 kb) which is highly conserved among Leptospira interrogans isolated in Korea was cloned into pBluescript vector from L. interrogans serovar lai WH20. The EcoRI fragment was sequenced, and a pair of primers (LP1 and LP2) was designed for PCR assay. PCR amplification of target DNA obtained from cultured L. interrogans showed that 274 bp could be detected when as little as 1...

a 9 bp deletion between cytochrome oxidase subunit ii and trna lys genes in mitochondrial dna, has proven to be an extremely informative marker for tracing population history. using the pcr-page and pcr-sscp techniques in this study, 152 dna samples collected from iranian populations were screened for the 9 bp deletion. no deletion was observed. a length polymorphism, most probably a 9 bp tripl...