The characteristics of growth, productivity and morphology varied with the mixing system and the applied agitation regime. In the first series of experiments, the flow characteristics of Aspergillus niger broth under different mixing conditions were analysed in a model bioreactor using RTS1 and ACS1. The kinetic energy E of flow fluctuations was measured in gassed and ungassed water and ferment...

background: enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-d-galactoside galactohydrolase, ec 3.2.1.23), trivial called lactase. orthogonal arrays design is an appropriate option for the optimization of biotechnological processes for the production of microbial enzymes. metho...

Aspergillus niger is usually considered to be a low virulence fungus, not commonly reported to cause invasive infections. Invasive pulmonary aspergillosis due to Aspergillus niger was diagnosed in a 43-year-old woman following bilateral lung transplantation. Intravenous voriconazole failed to control progression of the disease. Despite salvage therapy with a combination of voriconazole and casp...

A 67-year-old man who had been prescribed prednisolone for 20 months consulted our department due to repeated episodes of pneumonia. Computed tomography showed a large cavity in the apex of the right lung (Picture A, arrow). A fungal culture of a sample aspirated from the cavity revealed black-colored colonies (Picture B), which were later confirmed to be Aspergillus niger. A Grocott’s methenam...

The observation that the bitterest peptides from casein hydrolysates contain several proline residues led us to hypothesize that a proline-specific protease would be instrumental in debittering such peptides. To identify the desired proline-specific activity, a microbiological screening was carried out in which the chromogenic peptide benzyloxycarbonyl-glycine-proline-p-nitroanilide (Z-Gly-Pro-...

Aspergillus niger is a fungus of the genus Aspergillus. It has caused a disease called black mold on certain fruits and vegetables. The culture filtrates released from the A. niger ATCC 66566 were grown in Czapek dox broth (CDB) then filtered with flash chromatograph and were used for the bioassay after a growth of thirty days. The result demonstrated these mortalities with LC(50), LC(90), and ...

Hibiscus rosasinensis is a medicinal plant species which grows in moist soil favorable for the growth of Aspergillus niger (fungus) too. But extract of Hibiscus rosasinensis does n’t shows inhibition zone against Aspergillus niger in PDA (Potato Dextrose Agar) media in microbiological evaluation. In other hand, the Allium fistulosum root extract having antifungal activity shows moderate zone of...

Aspergillus niger is known for its efficient excretion machinery. However, problems have often arisen in obtaining high amounts of heterologous proteins in the culture medium. Here we present a quick method using sandwiched colonies to evaluate transgenic strains for secretion of heterologous proteins. Expressing the ABH1 hydrophobin of Agaricus bisporus in A. niger, we showed that low producti...

Filamentous fungi of the Aspergillus niger group are native soil saprophytic fungi. Industrial strains of this group have been extensively used for the production of plant degrading enzymes for the food and beverage, animal feed and paper-and-pulp industries. Recombinant DNA technology allows for the overproduction of these enzymes in copious amounts. The advantages and limitations of A. niger ...

A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable...