In order to observe the cytotoxicity of Acanthamoeba spp., which were isolated from contact lens containers as ethiological agents for the probable amoebic keratitis in Korea, the crystal violet staining method and LDH release assay were carried out. In the crystal violet staining method, among eight contact lens container isolates, isolate 3 (Acanthamoeba KA/LS5) showed 83.6% and 81.8% of cyto...

A simple, quantitative plate assay has been developed for use in the enumeration of Acanthamoeba. The technique uses an agarose overlay and low-nutrient medium to support the growth of amoebae on a bacterial lawn. The authors found that in this assay, individual trophozoites or dormant cysts will cause plaques to form in an Enterobacter aerogenes lawn. With the assay, it is possible to quantita...

A total of 36 strains belonging to 19 different species of Acanthamoeba were compared for temperature tolerance, ability to grow in an axenic medium, cytopathic effect in Vero cell culture, and virulence in mice. Pathogenic strains appeared to belong to different species, whereas pathogenic and nonpathogenic strains occurred in one species. Although growth at high temperatures and readiness to ...

background : acanthamoeba- bacteria interactions enable pathogenic bacteria to tolerate harsh conditions and lead to transmission to the susceptible host. the present study was aimed to address the presence of bacterial endosymbionts of acanthamoeba isolated from recreational water sources of tehran, iran. to the best of our knowledge this is the first study regarding occurrence of bacteria in ...

purpose: to present 2 cases of acanthamoeba keratitis diagnosed by confocal scanning before positive results of smear and culture, and their successful treatment with combined medical therapy and corneal epithelial debridment. patients and findings: confocal scan was carried out in 2 contact lens wearers with a clinical diagnosis of acanthamoeba keratitis followed by corneal epithelial debridme...

background: acanthamoeba genus is introduced as opportunistic and cosmopoli-tan parasite. monkey and wistar rat are appropriate models for experimental study on acanthamoeba infection. in this study acanthamoeba spp. were isolated from hot spring (hs), windows dust (wd) and a corneal sample of keratitis patient (kp) and their pathogenicity surveyed by in vitro and in vivo tests. methods: isolat...

conclusions a relatively high prevalence of acanthamoeba spp. in rivers reflects a risk alert for threatening human health in the region. however, well hygienic status of the tap waters considering acanthamoeba spp. cannot be ignored in western co-border regions of iran-iraq. this study can also serve as a platform for further explorations of water sources in iran and neighboring countries. res...

PURPOSE To describe the course and outcome of treatment of advanced Acanthamoeba sclerokeratitis with intravenous pentamidine. METHODS A case of advanced Acanthamoeba sclerokeratitis was resistant to conventional therapy and was treated with intravenous pentamidine. The eye was later removed due to incapacitating pain. RESULTS The eye showed Acanthamoeba organisms within the cornea and evid...

Free-living amoebae are widely distributed in soil and water. Small number of them was implicated in human disease: Acanthamoeba spp., Naegleria fowleri, Balamuthia mandrillaris and Sappinia diploidea. Some of the infections were opportunistic, occurring mainly in immunocompromised hosts (Acanthamoeba and Balamuthia encephalitis) while others are non opportunistic (Acanthamoeba keratitis, Naegl...

Since 1996, a new classification system has been used to assign members of the genus Acanthamoeba to different sequence types. This study aimed to place the thermophilic species Acanthamoeba jacobsi into the sequence type classification by sequencing part of the 18S rRNA gene for six isolates of this species. Phylogenetic analysis of the sequences showed the six strains to be closely related an...