UNLABELLED Results are presented supporting a regulatory role for the product of the MA3302 gene locus (designated MreA) previously annotated as a hypothetical protein in the methanogenic species Methanosarcina acetivorans of the domain Archaea. Sequence analysis of MreA revealed identity to the TrmB family of transcription factors, albeit the sequence is lacking the sensor domain analogous to ...

background: the opening and closing of the implantation window is important for successful pregnancy in eutherians. the recent study demonstrated that the window of uterine receptivity was prepared by the sole action of progesterone in mice, but the mechanism to close the window remained to be elucidated. methods: the pregnant mice were ovariectomized on the evening on the third day of pregnanc...

It has been demonstrated that radioactive lycopene results from the addition of labeled acetate to intact, green, ripening tomatoes (1, 2). In order to study more closely the metabolic pathway involved in the formation of this carotenoid, attempts to prepare a cell-free system which could synthesize lycopene were carried out. Labeled mevalonic acid could serve as a source of lycopene carbon in ...

An acetate-requiring mutant of Methanococcus maripaludis allowed efficient labeling of riboses following growth in minimal medium supplemented with [2-(13)C]acetate. Nuclear magnetic resonance and mass spectroscopic analysis of purified cytidine and uridine demonstrated that the C-1' of the ribose was about 67% enriched for 13C. This value was inconsistent with the formation of erythrose 4-phos...

Syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis has been identified as a significant anaerobic pathway in high-temperature (55°C) oil fields (1, 2). In a paper on acetate production from oil under sulfate-reducing conditions, Callbeck et al. (3) took note of that observation, only to emphatically state that “syntrophic acetate oxidation coupled to hydrogenotrophic methan...

Cobalt oxides, CoO and Co3O4, were obtained from Co (II) acetate tetrahydrate. The thermal decomposition pathway of the starting product was followed by combining thermogravimetric analysis in situ X-ray thermodiffraction. Under a nitrogen atmosphere, cobalt monoxide with Zn-blende rocksalt polymorphs could be almost as single phases at 330 400 °C, respectively. In addition to these oxyacetate,...

Anaerobic digesters rely on the diversity and distribution of parallel metabolic pathways mediated by complex syntrophic microbial communities to maintain robust and optimal performance. Using mesophilic swine waste digesters, we experimented with increased ammonia loading to induce a shift from aceticlastic methanogenesis to an alternative acetate-consuming pathway of syntrophic acetate oxidat...

Acetate kinase (ACK) catalyzes the reversible synthesis of acetyl phosphate by transfer of the γ-phosphate of ATP to acetate. Here we report the first biochemical and kinetic characterization of a eukaryotic ACK, that from the protist Entamoeba histolytica. Our characterization revealed that this protist ACK is the only known member of the ASKHA structural superfamily, which includes acetate ki...

In the study, we have examined the antitumor and antimicrobial activities of the methanol extract, the fractions, a fraction of total alkaloids and two alkaloids isolated from the stem of Erythroxylum caatingae Plowman. All test fractions, except the hexane fractions, showed antimicrobial activity on gram-positive bacteria and fungi. The acetate: methanol (95:5), acetate, chloroform and hexane ...

Knockout mice studies implicate the mammalian short-chain fatty acid (SCFA) receptors, FFAR2 and FFAR3- in colitis, arthritis and asthma. However, the correlation with human biology is uncertain. Here, we detected FFAR2 and FFAR3 expression in human monocytes via immunohistochemistry. Upon treatment with acetate SCFA or FFAR2- and FFAR3-specific synthetic agonists, human monocytes displayed ele...