Higher plants efficiently conserve energy ATP in cellulose biosynthesis by expression of sucrose synthase, in which the high free energy between glucose and fructose in sucrose can be conserved and used for the synthesis of UDP-glucose. A mixture of sucrose synthase and bacterial cellulose synthase proceeded to form UDP-glucose from sucrose plus UDP and to synthesize 1,4-beta-glucan from the su...

Calcofluor White ST, stilbene derivative used commerically as an optical brightener for cellulose, increased the rate of glucose polymerization into cellulose by resting cells of the gram-negative bacterium Acetobacter xylinum. This bacterium normally produces a ribbon of cellulose that is a composite of crystalline microfibrils. In concentrations above 0.1 mM, Calcofluor disrupts the assembly ...

The objective of this work is to verifying the consume of the minerals K, Na, Fe, Mg, P, S-SO4 (-2), B, N Total Kjedahl (NTK), NO3 (-)-N, and NH4 (+)-N in the production of bacterial cellulose by Acetobacter xylinum, according to the medium and the manner of cultivation. The fermentative process was in ripe and green coconut water. K and Na were determined by flame emission photometry, Mg and F...

The cytoplasmic and outer membranes of Acetobacter xylinum (ATCC 53582) were isolated by discontinuous sucrose density ultracentrifugation. Both lysozyme (EC 3.2.1.17) and trypsin (EC 3.4.21.4) were required for efficient crude membrane separation. Primary dehydrogenases and NADH oxidase were used as cytoplasmic membrane markers, and 2-keto-3-deoxyoctulosonic acid was used to identify the outer...

This study aimed to produce bacterial cellulose from paper waste sludge (PWS) as a method of utilizing the source remaining pulp in material. Initially, PWS was hydrolyzed by sulfuric acid create an enriched-reducing sugar hydrolysate. One-factor experiments were conducted with fixed amount (5 g) investigate influence hydrolysis conditions, including water, addition, temperature, and retention ...

Ryruvate-phosphate dikmase formation was induced on transfer of glucose-grown cells of Acefobacfer xylinum to succinateor pyruvate-containing media. Enzyme formation did not occur if such media were supplemented with glucose or in the presence of chloramphenicol. Fructose and glycerol were less effective in repressing enzyme formation. When glucose was added to cultures of cells growing on succ...

The synthesis of cellulose by washed suspensions of Acetobatter xylinum proceeds in the presence of iodoacetate at concentrations which completely inhibit the oxidation of glyceraldehyde-a-phosphate (1). Since glucose was the only carbon compound added, the possibility was considered that the energy required for the biosynthetic process may be derived from reactions linked to the pentose phosph...