Note In 1979, a large molecular weight complex composed of adenosine deaminase (ADA) were reported to be found as an adenosine deaminase-binding protein (ADBP), also known as adenosine deaminase complexing protein-2 (ADCP2). In 1993, this adenosine deaminase-binding protein is determined to be identical to CD26, a T-cell activation molecule and a 110-kD glycoprotein that is present also on epit...

The activity of adenosine deaminase (ADA) was measured in 62 discrete regions of the CNS, and in some autonomic and sensory ganglia, peripheral nerves, and peripheral tissues of the rat using an automated high-pressure liquid chromatography (HPLC) method. The formation of inosine and hypoxanthine as a measure of ADA activity in homogenates of brain was optimal at pH 7.0, linear for up to 60 min...

Background: Measurement of pleural fluid adenosine deaminase (ADA) and Lactate dehydrogenase (LDH) enzymes activity has gained increasing popularity as a diagnostic test for tuberculous and non-tuberculous pleuritis, especially in countries where the prevalence of TB is high. It carries a high sensitivity, inexpensive and easy. Objective: To demonstrate the diagnostic value of increased level o...

In order to investigate purin and primidin metabolism pathways in hepatitis, adenosine deaminase (ADA) and guanosine deaminase (GDA) activities in sera of patients with different types and manifestations of viral hepatitis disease (A, B, C, D, E, chronic, acute) were investigated and compared with the control group of healthy individuals. Hepatitis cases were classified with respect to their se...

Mice deficient in the enzyme adenosine deaminase (ADA) have small lymphoid organs that contain reduced numbers of peripheral lymphocytes, and they are immunodeficient. We investigated B cell deficiency in ADA-deficient mice and found that B cell development in the bone marrow was normal. However, spleens were markedly smaller, their architecture was dramatically altered, and splenic B lymphocyt...

The biochemical mechanisms by which a genetically determined deficiency of adenosine deaminase leads to immunodeficiency are still poorly understood and prompted this study. We have examined the effects of the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine hydrochloride (EHNA) upon the response of human peripheral blood mononuclear cells to the mitogen concanavalin A (Con A...

Genes located outside the HLA region (6p21) have been considered as candidates for susceptibility to ankylosing spondylitis. We tested the hypothesis that the G22A polymorphism of the adenosine deaminase gene (ADA; 20q13.11) is associated with ankylosing spondylitis in 166 Brazilian subjects genotyped for the HLA*27 gene (47 patients and 119 controls matched for gender, age and geographic ...

Possible effects of aqueous garlic (Allium sativum Linn.) and red clover (Trifolium pratense Linn.) extracts on adenosine deaminase (ADA) activities were investigated in cancerous and non-cancerous human liver tissues and results were compared with those of Fludarabine, a known ADA inhibitor. Ten cancerous and 10 non-cancerous adjacent liver tissues were obtained from patients with metastatic t...

The aim of the study was to explore the usefulness of adenosine deaminase (ADA) activity as a marker of colon tumor progression and to find a quantitative expression of ADA in mucosa of colon carcinoma, polyps, adjacent to tumors, or mucosa from anastomosis after surgical resection. The highest ADA activity was obtained from mucosa adjacent to carcinoma compared to healthy tissue. The ADA activ...

We studied an Arab family in which two infants died of severe combined immunodeficiency caused by adenosine deaminase (ADA) deficiency. One infant had purine nucleoside phosphorylase (PNP) activity in the leucocytes only half that of normal. Four other infant siblings had previously died from infections before the age of 2 months. Hyperpigmented skin lesions preceded death in three cases. The h...