The Aedes aegypti densovirus (AeDNV) has potential as a delivery vector for foreign nucleic acids into mosquito cells. In this study, we investigated the ability of plasmids containing recombinant viral transducing genome to induce RNA interference (RNAi) effects in C6/C36 cells. We then evaluated the efficiency of a recombinant AeDNV vector to induce RNAi in Aedes albopictus larvae. We found t...

Five media, formulated from the seeds of five legume varieties, dried cow blood, and mineral salts, were assessed for the growth and production of insecticidal properties of Bacillus thuringiensis subsp. israelensis. Bacterial powders prepared from the broth cultures were assayed against the larvae of Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae. A standard primary powder of B. ...

This is the description of how nine Aedes aegypti larvae were found in a natural breeding site in the Pinheiros neighborhood, city of Sao Paulo, SP, Southeastern Brazil. The record was conducted in December 2014, during an entomological surveillance program of dengue virus vectors, with an active search of potential breeding sites, either artificial or natural. FindingAe. aegypti larvae in a tr...

Sublethal concentrations of Abate (temephos) were applied to F2 generation Aedes aegypti larvae, and fecundity and longevity were recorded in the emerged adults. Females exposed to Abate oviposited only in the first 2 gonotrophic cycles, meanwhile control females laid a few eggs after taking the third blood meal. Dosages of 0.009, 0.013 and 0.015 mg/liter of Abate decreased the mean egg product...

The invasive container-dwelling mosquito Aedes albopictus (Skuse) shows modest behavioral responses to water-borne cues from predatory Corethrella appendiculata Grabham in North America. We investigate whether Ae. albopictus adjust their antipredatory responses to be proportional to size-dependent risk of predation. Fourth-instar Ae. albopictus attain a size refuge from C. appendiculata predati...

The insecticide susceptibility of Aedes aegypti adults and larvae from Santo Domingo, Dominican Republic, was investigated using World Health Organization standard procedures. A field strain was more resistant to insecticides than a colony strain that originated from the same place. Larvae produced from ovitrap-collected eggs were resistant to temephos (78.2% mortality on exposure to 0.025 mg/l...

Aedes aegypti (Linnaeus) and Aedes albopictus Skuse mosquitoes transmit serious human arboviral diseases including yellow fever, dengue and chikungunya in many tropical and sub-tropical countries. Females of the two species have adapted to undergo preimaginal development in natural or artificial collections of freshwater near human habitations and feed on human blood. While there is an effectiv...

A monitoring of male and larvae of mosquitoes was conducted during 1991-1994, at the Irrigation Project in Arenal-Tempisque, Guanacaste, Costa Rica. CDC CO2-baited traps were used to collect adults of mosquitoes and dips were used for immatures of culicids. A total of 1,480 larvae and 1,129 males of culicids were identified resulting in, Aedes with 6 species, Anopheles, Mansonia and Psorophora ...

When exposed to concentrations of less than one Plagiorchis noblei cercariae per cc of water, the acquisition of metacercariae by Aedes aegypti larvae increased significantly with each successive instar but declined precipitously upon pupation. Thus, 1st instar larvae acquired no metacercariae, whereas 4th instars had a mean abundance of 6.15: pupae only acquired a mean of 0.62 parasites. Paras...

The effect of relative humidity (43%, 75%, 86% and > 98%) on Aedes aegypti eggs treated with Metarhizium anisopliae or water only was tested for up to a six months exposure at 25 degrees C. Survival of larvae inside eggs was clearly affected by the lowest humidity (43%) tested, and eclosion diminished at all humidities after increasing periods of exposure. M. anisopliae showed to have a strong ...