The fertility (positive ultrasound scanning 60-75 days after insemination) of ram spermatozoa frozen in Tris- or zwitterion/glucose/egg yolk/glycerol-based diluents was examined during the natural breeding season (autumn). In Experiment 1 Tris-citrate, high glucose and Hepes-NaOH diluents were used and ewes (n = 266) were inseminated (induced oestrus) with 20 (uterus) or 180 (cervix) million mo...

OBJECTIVE To assess on which day to cryopreserve and transfer thawed embryos in good-responder patients by comparing the cycle outcomes of day 3 transfers vs blastocysts formed through extended culture before or after cryopreservation. DESIGN Retrospective clinical study. SETTING Private IVF center. PATIENT(S) Frozen-thawed cycles (n = 2,531) who had ETs at day 3, 5, and 6 and post-thawed...

THREE MODES FOR CRYOPRESERVATION (CP) OF HUMAN IPSC CELLS HAVE BEEN COMPARED: STD: standard CP of small clumps with 10% of CPA in cryovials, ACC: dissociation of the cells with Accutase and freezing in cryovials, and PLT: programmed freezing of adherent cells in plastic multiwell dishes in a programmable freezer using one- and multistep cooling protocols. Four CPAs were tesetd: dimethyl sulfoxi...

OBJECTIVES To translocation (externalization) of phosphatidylserine lead at least the five negative effects observed during cells cryopreservation: hypoxia, increasing of intracellular Ca2+, osmotic disruption of cellular membranes, generation of reactive oxygen species (ROS) and lipid peroxidation. The aim of this study was to test the intensiveness of the phosphatidylserine translocation imme...

Abstract The Mu Us Desert in China is significantly affected by seasonal freeze–thaw processes. In order to evaluate the adaptation potential of reconstituted soil with different proportions feldspathic sandstone and sand extreme environment, laboratory simulation freeze-thaw experiments was conducted study characteristics C N under conditions. results showed that content organic matter reached...

The influence of thawing procedures on fertility of semen in .25-ml Continental 3 straws was investigated in a series of eight trials utilizing 1,634 cows. The following pregnancy rates were obtained: Trial 1, 38.6 vs 23.0% (P<.05) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 2, 29.7 vs 22.2% (P>.10) for semen thawed in 75 C water/7 sec vs "in the cow"; Trial 3, 82.8 vs 61.6% (P<...

BACKGROUND The availability of an efficient cryopreservation program is especially important in the case of embryos that have undergone blastomere biopsy for PGD. Unfortunately, the freezing/thawing of biopsied embryos has given disappointing results when performed at the cleavage stage. In this study, embryos diagnosed as normal after PGD were grown to the blastocyst stage, frozen and thawed f...

Our investigations focused on spindle dynamics/displacement in frozen-thawed human oocytes. In each oocyte, prior to freezing and after thawing and culturing, the presence/location of the spindle was determined with the Polscope technique. A total of 259 oocytes have been thawed with a survival rate of 81.1%. From the 210 survived oocytes, 165 were fertilized (78.6%) and 89.1% of them cleaved. ...

BACKGROUND The aim of our experimental work was to assess morphological changes of arterial wall that arise during different thawing protocols of a cryopreserved human aortic root allograft (CHARA) arterial wall. METHODS The experiment was performed on CHARAs. Two thawing protocols were tested: 1, CHARAs were thawed at a room temperature at +23°C; 2, CHARAs were placed directly into a water b...

Microbial release of greenhouse gases from thawing permafrost is a global concern. Seventy-six metagenomes were generated from low-soil-organic-carbon mineral cryosols from Axel Heiberg Island, Nunavut, Canada, during a controlled thawing experiment. Permafrost thawing resulted in an increase in anaerobic fermenters and sulfate-reducing bacteria but not methanogens.