Screening for pathogenic Vibrio parahaemolyticus has become routine in certain areas associated with food-borne outbreaks. This study is an evaluation of the CHROMagar Vibrio (CV) medium-PCR protocol and the conventional method (TCBS (thiosulfate-citrate-bile salts-sucrose) agar plus biochemical and Wagatsuma agar tests) for detection of V. parahaemolyticus in shrimp, water, sediment, and stool...

Total coliform counts obtained by means of standard membrane filtration techniques, using MacConkey agar, m-Endo LES agar, Teepol agar, and pads saturated with Teepol broth as growth media, were compared. Various combinations of these media were used in tests on 490 samples of river water and city wastewater after different stages of conventional purification and reclamation processes including...

E. coli O157:H7 is recognised as an important human pathogen world-wide and has been associated with diseases such as haemorrhagic colitis (HC), haemolytic uraemic syndrome (HUS) and thrombotic thrombocytopaenic purpura (TTP). Accurate laboratory detection of E. coli O157:H7 is important for diagnostic purposes and to justify epidemiological data on E. coli O157:H7. A well-known phenotypic char...

this study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°c in dark conditions. the results showed that different treatments had a significant effect on the germination percentage. the...

ascochyta rabiei is one of the devastaining fungi pathogens of chick-pea (cicer arietinum l.) crops. in the present investigation, forty-two iranian isolates of ascochyta rabiei fungi (including highly, weakly and moderate virulent isolates), whose pathogenisities were determined previously, were studied. polygalacturonase is a main factor in the initiation of ascochyta blight disease. therefor...

Lactic acid bacteria have been used for many years as natural bio preservatives in fermented foods and are also believed to have beneficial health effects on the host. However selection of strain is an important criteria which is mainly based on evaluating the physiological properties of the strain. In this study, lactic acid bacteria were isolated from various dairy, non-dairy and human source...

objective: n present study effect of various factors including sucrose, agar, peg, agno3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties dom siah and nemat. methods: 330 explant (mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction murashige and skoog (ms) medium (murashige and skoog 1962) s...