A large set of genome-wide markers and a high-throughput genotyping platform can facilitate the genetic dissection of complex traits and accelerate molecular breeding applications. Previously, we identified about 0.9 million SNP markers by sequencing transcriptomes of 27 diverse alfalfa genotypes. From this SNP set, we developed an Illumina Infinium array containing 9,277 SNPs. Using this array...

The objectives of this study were to expand the database and determine the intestinal digestibility of rumen undegradable protein (dRUP) of common dairy feeds and to determine the effects of feeding 37.9% wet corn gluten feed on these estimates. Two ruminally and duodenally fistulated steers were assigned randomly to a crossover design with 4-wk periods. The mobile bag technique was used to det...

Building a 2,000-square-foot house leaves about a ton of gypsum wallboard waste from end cuts, window and door cutouts, and broken boards. In the United States, three million tons of gypsum wallboard waste is dumped in landfills each year. In addition to depleting space, microbial action can decompose gypsum to malodorous hydrogen sulfide gas, which at high enough exposures can cause irritation...

In the present study, field surveys were conducted to detect potential associational susceptibility of crop plants (potato, alfalfa and maize) caused by Canadian goldenrod, Solidago canadensis (L.), one most common invasive weeds in Central Eastern Europe, via plant associated virus vectoring aphids. Assessments made two major agricultural land types: crops grown under high input vs. low-input ...

Molting is a process by which a hen's reproductive tract is rejuvenated prior to the beginning of a laying cycle. This process is often artificially induced in commercial settings in order to extend the productive life of a flock of hens. The most common method for the induction of molt is feed withdrawal for a period of several days. It has been noted that feed withdrawal, while effective in i...

Cold acclimation in alfalfa (Medicago sativa L.) plays a crucial role in cold tolerance to harsh winters. To examine the cold acclimation mechanisms in freezing-tolerant alfalfa (ZD) and freezing-sensitive alfalfa (W5), holoproteins, and low-abundance proteins (after the removal of RuBisCO) from leaves were extracted to analyze differences at the protein level. A total of 84 spots were selected...

This paper reviews the current status, methodology, achievements, and prospects of alfalfa (Medicago sativa L.) breeding in China. There are 77 cultivars that have been registered in the country, these include 36 cultivars bred through breeding programs, 17 introduced from overseas, 5 domesticated from wild ecotypes, and 19 through regional collection/breeding programs. Cultivars have been sele...

Alfalfa hay is, or should, be highly valued as a dairy cattle feed due to its relatively high (for a forage) crude protein (CP) level which has a low rumen soluble fraction and a relatively high rumen escape fraction which contains a high level of the key amino acid lysine. It should also be valued for its high level of rapidly rumen digested pectin which has a high cation exchange capacity (CE...

Winter damage, especially in northern climates, is a major limitation of the utilization of perennial forages such as alfalfa. Therefore, improving freezing tolerance is imperative in alfalfa genetic breeding. However, freezing tolerance is a complex trait that is determined by many genes. To understand the complex regulation mechanisms of freezing tolerance in alfalfa, we performed small RNA s...

Alfalfa (Medicago sativa L.) is a highly attractive plant host to Lygus spp. and is used as a trap crop in California organic strawberries to influence the dispersion and dispersal of these pests, particularly Lygus hesperus Knight. The abundance and distribution of Lygus spp. nymphs between two trap crops separated by 50 strawberry rows was analyzed in 2008 and 2010. Nymphs demonstrated a bimo...