To increase cell concentration and the production of alkaline protease, fed-batch cultures with standard medium (1x) or concentrated medium (2x or 5x) as feed were performed. In a fed-batch culture with standard medium as feed, the biomass concentration reached 9.6 g L–1 and the protease activity obtained was 8500 U mL–1 compared to 3000 U mL–1 obtained by growing Teredinobacter turnirae cells ...

Natural senescence is associated with several fold increase in activity of protease and is characterized by the degradation and loss of proteins therefore plant proteases have been a common studied interestingly. Protease from senesced leaves of the papaya plant (Carica Papaya) was partially purified by ammonium sulfate precipitation. It showed 15 fold purification. Optimum protease activity wa...