Alkalophilic bacilli maintain a cytoplasmic pH that is lower than optimal external pH values. The current study, using isolated membrane vesicles from Bacillus alcalophilus, indicates that respiration results in proton extrusion as in other organisms. Cation/proton antiporters then catalyze inward proton movements. Upon energization with ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine, Na'-l...