We have labeled the nucleoside triphosphate-binding domain of Escherichia coli rho factor with the ATP affinity analog [3H]pyridoxal 5'-diphospho-5'-adenosine (PLP-AMP). PLP-AMP completely inactivates the RNA-dependent ATPase activity of rho upon incorporation of 3 mol of reagent/mol of hexameric rho protein. Although the potency of PLP-AMP is enhanced when an RNA substrate such as poly(C) is p...

The binding of AMP to rabbit muscle glycogen phosphorylase a (EC 2.4.1.1.) has been studied by equilibrium dialysis and isothermal microcalorimetry at pH 6.9 over a temperature range of 25 degrees C to 35 degrees C. Thermal titration experiments were carried out in various buffer systems. We have found by these methods that a certain number of protons are released when the protein binds to the ...

Abstract. Understanding concentration–discharge (C–Q) relationships can inform catchment solute and particulate export processes. Previous studies have shown that the extent to which baseflow contributes streamflow affect C–Q in some catchments. However, current understanding on effects of contribution shaping patterns is largely derived from temperate As such, we still lack quantitative these ...

Ultra-high temperature ceramics (UHTCs) are most recently getting much attention for structural parts of hypersonic missiles with their cruising speed more than Mach 5. Most the UHTCs poor sinterability carbides, nitrides, and borides. Therefore, they have been studied developed a long time. However, there still many problems to solve. In this paper, based on solid-state reaction presented as a...

May we have this dance? Celebrations are afoot at C&EN. While the magazine marks its centennial, Newscripts also has a special anniversary: for 80 years, we’ve been bringing C&EN readers quirky science news. To paraphrase late Kenneth M. Reese, who wrote from 1968 to 2004, column favors chemical over nonchemical, scientific nonscientific, peculiar pedestrian. Readers interested in Newscripts’ o...

Cyclic dinucleotides such as bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) and bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) represent important second messengers in bacteria. Although their synthesis has not been described in plants so far, they may be involved in the regulation of bacterial phytopathogen-plant interactions as well as rhizobium plant symbiosis. ...

Here we describe the presence of two very similar but unusual variants of AmpC cephalosporinase in each Cronobacter sakazakii and C. malonaticus isolates conferring resistance exclusively to first generation cephalosporins. During a survey on the antibiotic resistance patterns of C. sakazakii and C. malonaticus strains isolated from a milk powder production facility, originally two different ph...

Infection with Listeria monocytogenes strains that enter the host cell cytosol leads to a robust cytotoxic T cell response resulting in long-lived cell-mediated immunity (CMI). Upon entry into the cytosol, L. monocytogenes secretes cyclic diadenosine monophosphate (c-di-AMP) which activates the innate immune sensor STING leading to the expression of IFN-β and co-regulated genes. In this study, ...