α-amylase inhibitors are used in the treatment of type II diabetes mellitus. This study aims to identify the α-amylase inhibitors from two medicinal plants Mentha piperita and Annona squamosa. Primarily, the extract of both leaves was prepared using methanol and water and perform the screening of phytochemicals constituents present in the extract. Aqueous and methanol extract of mentha leaves c...

the aim of this study was production and partial purification of α-amylase enzyme by bacillus licheniformis. b. licheniformis was allowed to grow in broth culture for purpose of inducing α-amylase enzyme. optimal conditions for amylase production by b. licheniformis are incubation period of 120 h, temperature of 37 °c and ph 7.0. the α-amylase enzyme was purified by ion exchange chromatography ...

willow leaf beetle, plagiodera versicolora is an important pest of willow trees that feeds on the leaves both as larvae and as adults. -amylases (ec 3.2.1.1) are the major insect digestive enzymes that catalyze the endohydrolysis of long -1,4-glucan chains such as starch and glycogen. in the current study, -amylase activity was studied in the midgut of larvae and adults of p. versicolora. am...

Seed germination plays important roles in the establishment of seedlings and their subsequent growth; however, seed germination is inhibited by salinity, and the inhibitory mechanism remains elusive. Our results indicate that NaCl treatment inhibits rice seed germination by decreasing the contents of bioactive gibberellins (GAs), such as GA1 and GA4, and that this inhibition can be rescued by e...

Exocrine pancreatic secretion stimulated by vasoactive intestinal polypeptide (VIP), which acts through the adenylyl cyclase-cAMP pathway, is potentiated by stimulation with other secretagogues such as CCK and carbachol (CCh). However, the potentiating effect is abolished by the same secretagogues at supramaximal concentrations. In the present study, we examined the mechanisms by which supramax...

The inhibitory potential of purified α amylase inhibitors of sorghum on the digestive enzyme of Tribolium Castaneum, a devastating insect pest of economic importance have been studied. Qualitative and quantitative differences in α amylase of Tribolium Castaneum upon feeding on natural and artificial diets were evident. Natural diets were legumes like Black gram, Pigeon Pea, Red lentil, Cowpea a...

The nano sized copper oxide material (CuONPs) were prepared by the greener way and an approach functionalized polymer using Sarcostemma acidum stem extracts. in-vitro anti-diabetic activity was studied through α-glucosidase α-amylase inhibitory methods demonstrated in stem, biological synthesized CuONPs polymerized CuONPs. percentage inhibition at 100 μg/ml, 200 300 400 μg/ml 500μg/ml concentra...

AIM To validate plant species for hypoglycaemic activity. MATERIALS AND METHODS Four plants were investigated for hypoglycaemic activity by evaluating inhibiting effects on carbohydrate-hydrolising enzymes: alpha-glucosidase and alpha-amylase. Acetone plant extracts were screened against C2C12 myocytes, 3T3-L1 preadipocytes and Chang liver cells by measuring glucose uptake. Cytotoxicity was d...

Pancreas is the primary organ involved in sensing the organism’s dietary and energetic states via glucose concentration in the blood and in response to elevated blood glucose, insulin is secreted. Diabetes mellitus is a metabolic disorder which results from defects in insulin secretion. Among the various methods of Diabetes management, herbal products are best alternative drug with zero side-ef...

Midgut hydrolytic enzymes of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were identified and partially characterized. Km, Vmax, optimum pH, and specific activity were determined for proteolytic enzymes and αamylases. All hydrolytic enzyme activity had an optimum pH value in the alkaline pH range. We observed major serine protease activity, together with minor cysteine-like activity, th...