The propagation of oil palm seeds through tissue culture and somatic embryogenesis is a preferred method due to its ability produce entire plants without the need for gamete fusion. While embryo proliferation typically performed on solid media, this approach less efficient than liquid which both more cost-effective can lead better results. Thus, study aimed determine most effective combination ...

New Plant Breeding Techniques (NPBTs) protocols have been developed to produce new grape varieties with improved quantitative and qualitative characteristics. Reliable transformation for grapes are based on the generation/induction of embryogenic callus cells that then transformed. Varieties such as Italia proven be very recalcitrant regeneration via somatic embryogenesis. In this work, develop...

In order to simplify the experimental procedure and treatment procedure, we preserved embryonic callus (EC) of Fraxinus mandshurica more efficiently. this paper, established a method for cryopreservation EC F. by vitrification. was subcultured 7–10 days (d). Vigorous with good growth conditions were selected, performed The best pre-culture on 0.5 mol·L−1 sucrose medium 3 d, load culture in liqu...

Root-knot nematodes (RKNs; Meloidogyne spp.) induce feeding cells (giant cells; GCs) inside a pseudo-organ (gall) from still unknown root cells. Understanding GCs ontogeny is essential to the basic knowledge of RKN-plant interaction and to discover novel and effective control strategies. Hence, we report for the first time in a model plant, Arabidopsis, molecular, and cellular features concerni...