Species in the genus Castanea Mill. are one of the worthwhile species in the Hyrcanian forest of west Guilan (N Iran) with low region such as Siahmazgi, Ghalerodkhan and Visrod. that do not have good condition by reason of human interfering and disease. They have varying economic importance as nut tree crops, therefore, DNA barcoding technique can be a straightforward method for taxonomic statu...