Sera of rabbits immunized against Salmonella typhi have been studied for the idiotypy of certain of their components, i.e., the property of these components to possess an antigenic specificity which is different in individual rabbits, and which varies with the antigens against which these rabbits have been immunized. The reagent used (precipitating anti-idiotypic sera) have been prepared by inj...

Antisera to hCG were incubated with living human spermatozoa. Agglutination and complement-dependent immobilization of the spermatozoa occurred with the anti-hCG sera but not with control sera. The antisera also reacted with human blood cells. The anti-sperm activities were largely removed by absorbing the sera with human blood cells, although these absorptions had no apparent effect on the ant...

OBJECTIVE To evaluate whether a new fluid-phase filtration radioassay possesses both high sensitivity and specificity compared with the currently used ELISA and Farr assays. METHODS Sequential sera (25 samples) from 9 patients with systemic lupus erythematosus (SLE), sera from 20 patients with SLE possessing anti-dsDNA antibodies by the Crithidia assay, 75 patients with rheumatoid arthritis p...

Background & Aims: Influenza viruses are important causes of mortality and morbidity in children. The aim of this study was to assess the presence of antibodies (IgG) against Influenza A in children in Kerman, Iran. Methods: In a cross-sectional study, 200 children aged 4-14 years referred to Besaat Clinic and Afzalipour hospital for diseases other than influenza were enrolled. Sera were tested...

Serological markers of hepatitis A and B virus infection were determined by radioimmunoassay in 162 university students. Antibody to hepatitis B e antigen (anti-HBe) was demonstrated in 11 of 15 students positive for both antibody to hepatitis B e antigen (HBsAg) and antibody to hepatitis B core antigen (anti-HBc). The presence of anti-HBc alone seemed to denote previous hepatitis B virus infec...

The anti-pre-S antibody in the samples of sera from normal healthy persons and patients with different clinical types of liver diseases due to hepatitis B virus (HBV) infection was detected by a newly established enzyme-linked immunosorbent assay technique. This test is a blocking assay where anti-pre-S antibody in the patient's serum blocks subsequent addition of horse radish peroxidase-labell...

Twenty-five patients with endocarditis and serum antiglobulin activity were studied. The antiglobulins were detected by use of the latex agglutination reaction; only eight sera reacted with sensitized sheep cells and one with human O CD erythrocytes coated with anti-CD (Ripley) antibody. Absorption of the sera with the infecting organisms produced a decrease in antiglobulin activity in 10 of th...

INTRODUCTION We aimed to evaluate the prevalence of "anti-HBc alone" among Syrian blood donors, highlighting the possibility of representing occult HBV infection. METHODOLOGY Sera of 3,896 healthy blood donors were tested for both HBsAg and anti-HBc. HBsAg-negative, anti-HBc-positive samples were further tested for the antibodies to HBsAg (anti-HBs), and "anti-HBc alone" sera were tested for ...

background: sen virus (senv) is the latest virus proposed as a cause of unknown hepatitis cases. among nine detected genotypes of the virus, genotypes d and h are more frequent in hepatitis cases of unknown origin. the aim of this study was to determine the frequency of senv-d and senv-h genotypes in the sera of healthy individuals and hepatitis b and c patients. methods: totally, 200 serum sam...

Toxoplasma gondii causes congenital toxoplasmosis in newborns resulting with fetal anomalies. Determining the initiation time of infection is very important for pregnant women and current serological assays have drawbacks in distinguishing the recently acute toxoplasmosis. Diagnosis of recently acute infection may be improved by using stage specific antigens in serological assays. In the presen...