BACKGROUND Although lung transplantation from donation after cardiac death (DCD), especially uncontrolled DCD, is limited by warm ischemic periods, the molecular mechanism of warm ischemia-reperfusion-injury (IRI) has not been well elucidated. The purpose of this study was to clarify the particular longitudinal mechanisms of molecular factors involved in warm IRI. METHODS Cold ischemic-time (...

Cytotoxic T lymphocyte antigen 4 (CTLA4) appears to negatively regulate T cell activation. One mechanism by which CTLA4 might antagonize T cell function is through inhibition of CD28 signaling by competing for their shared ligands B7-1 and B7-2. In addition, CTLA4 ligation could initiate a signaling cascade that inhibits T cell activation. To address whether CTLA4 could inhibit immune responses...

NO derived from iNOS has been implicated in cardiac rejection. However, little is known about the role of the reactive nitrogen species, peroxynitrite. We examined the protective actions of a peroxynitrite decomposition catalyst WW85 in an experimental model of acute cardiac rejection. Heterotopic, abdominal transplantation of rat donor hearts was performed. Groups included: isografts, allograf...

Harvest of furbearers through trapping has been challenged by anti-trapping organizations for centuries, with organizational goals often including prohibition of all forms of trapping. Challenges to trapping may also include dissention among state wildlife agencies, pro-hunting organizations, and pro-trapping organizations. Despite recent efforts by anti-trapping organizations and occasional di...

BACKGROUND Anti-CD154 (MR1) monoclonal antibody (mAb) and rapamycin (RAPA) treatment both improve survival of rat-to-mouse islet xenograft. The present study investigated the effect of combined RAPA/MR1 treatment on rat-to-mouse islet xenograft survival and analyzed the role of CD4(+)CD25(+)Foxp3(+) T regulatory cells (Treg) in the induction and maintenance of the ensuing tolerance. METHODOLO...

The CD40-CD40 ligand (CD40L) interaction is a key event in the initiation of an adaptive immune response, and as such the therapeutic value of CD40L blockade has been studied in many experimental models of tissue transplantation and autoimmune disease. In rodents, transplantation of allogeneic tissues under the cover of anti-CD40L Abs has resulted in prolonged graft survival but not tolerance. ...

BACKGROUND Coronary vein graft disease is an important contributor to the morbidity after coronary artery bypass grafting (CABG). Late occlusion of the graft is a serious complication that limits the use of the saphenous vein as a coronary bypass conduit. It is frequently encountered in old, degenerated vein grafts with advanced atherosclerotic plaque formation. Paraoxonase-1 (PON-1) is an HDL-...

Kidney allografts are frequently lost due to alloimmunity. Still, the impact of early acute rejection (AR) on long-term graft survival is debated. We examined this relationship focusing on graft histology post-AR and assessing specific causes of graft loss. Included are 797 recipients without anti-donor antibodies (DSA) at transplant who had 1 year protocol biopsies. 15.2% of recipients had AR ...

Abstract Background The clinical significance of de novo donor-specific antigen (DSA) in renal transplant recipients is not yet fully understood. This study aimed to report the prevalence DSA detected antihuman leukocyte (HLA) antibody testing and evaluate association between prognosis living-donor at our hospital. Methods Of 110 patients who underwent transplantation from 1980 2019, 80 anti-HL...