The loss of tyrosinase, the key enzyme in melanin synthesis, has been implicated in the dedifferentiation of malignant melanocytes. The presence of tyrosinase transcripts and antigenic peptides in melanoma tumors prompted us to investigate whether the basis for the loss of the enzyme was proteolytic degradation. Toward this aim, we followed the kinetics of synthesis, degradation, processing, ch...

In this study, total phenols (TP) and proanthocyanidins (PAs) in the leaves branches of Cinnamomum camphora were investigated, isolated PAs determined using reversed-phase high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS). The anti-tyrosinase antioxidant activities also evaluated. Furthermore, scanning study dihydroxyphenylalanine (L-DOPA) oxidation ...

Abstract Seaweeds have been identified as a valuable source of natural ingredients and their use in cosmetics is being studied increasingly. This work sought to understand the possible uses three species brown algae present along Portuguese coast: Bifurcaria bifurcata , Saccorhiza polyschides Fucus spiralis . Considering idea more sustainable resources, two extracts (water (W) ethanol (E)) frac...

BACKGROUND Ultraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders. Novel whitening and anti-wrinkle compounds from natural products have recently become of increasing interest. The purpose of this study was to find products that reduce ROS in 14 Thai plant extracts. METHODS To determine total phe...

Fermentation is a traditional technique used to increase nutrients, flavonoids, vitamins, minerals, and the flavor of raw materials. In this study, adlay bran was fermented by Lactobacillus brevis MJM60390 (FAB), anti-melanogenic effect investigated. The results demonstrated that FAB significantly suppressed melanin accumulation in mouse melanogenic B16F10 cells, activity higher than non-fermen...

The present study aimed to evaluate the anti-melanogenic activity of 1,6-diphenyl-1,3,5-hexatriene and its derivatives in B16F10 murine melanoma cells and zebrafish embryos. Twenty five (1E,3E,5E)-1,6-bis(substituted phenyl)hexa-1,3,5-triene analogs were synthesized and their non-cytotoxic effects were predictively analyzed using three-dimensional quantitative structure-activity relationship ap...

An amperometric biosensor for the determination of catechol was developed by immobilizing tyrosinase (tyr) on gold nanoparticles (AuNPs) and a (3-mercaptopropyl)-trimethoxysilane (MPTS) sol-gel three-dimensional network film-modified gold electrode. The AuNPs self-assembled in a sol-gel network provided an excellent microenvironment for an enzymatic reaction between tyrosinase and the substrate...