A monoclonal antibody that recognizes antigenic determinants on the nucleus of cultured mammalian cells was isolated. Immunofluorescence studies using this antibody showed that the recognized antigen was present not only on the nucleus but also in cytoplasmic vesicles of interphase cells and in the perichromosomal region of mitotic cells. Premature chromosome condensation analysis showed that t...

background and aims: the contribution genetic and antigenic diversity of h9n2 influenza viruses in evading from immune responses, cytotoxic t lymphocytes (ctl) epitopes in hemagglutinin (ha) protein restricted by hla binding peptides was identified. materials and methods: phylogenetic analyses were carried out for all of full length ha and deduced amino acid sequences of h9n2 viruses available ...

We compared the E2-HVR1 region in HCV-1b positive B-NHL cases from a multicenter study with sequences from studies related to lymphoproliferative disorders and B cell compartmentalisation. We found rare and unique mutations both in B-NHL isolates and in cases with lymphoproliferative disorders and lymphocyte infection. These rare mutations could have an important effect on HVR1 region and, as a...

The immunodominant region of the human herpesvirus 8 (HHV-8), the antibody-binding site of glycoprotein K8.1A, was mapped to the N-terminal region by using overlapping peptides and a residue replacement method. The main epitope was located within residues 44 to 56 (GQVYQDWL----C). Based on this information, we developed an enzyme immunoassay to detect HHV-8 antibodies in human sera using a four...

A population of polysomes isolated from frogskinis capable of supporting protein synthesis in a cell-free system containing an energy generating system,' soluble components', and amino acids. These polysomes catalyse the oxidation of DOPA after gentle trypsinization, and they also have antigenic determinants attributable to tyrosine oxidase. Skin polysomes sedimented in 10-30 % sucrose gradient...

Antigenic variation of type A foot and mouth disease (FMD) virus in Thailand was examined using a total of 82 field viruses isolated between 1986 and 1989. A two-dimensional serum microneutralisation test was used to compare these isolates to a reference strain, A15 Bangkok 1960 (A BKK/60). Viruses regarded as unrelated to A BKK/60 were compared to another reference strain, A22 Nakhon Pathom 19...

About 50% of patients with the polymyositis-scleroderma overlap syndrome are reported to have autoantibodies to a nucleolar particle termed PM/Scl. The particle consists of several polypeptides of which two proteins of 75 and 100 kD have been identified as the major antigenic components. Here we report on the cDNA cloning and partial epitope mapping of the 100-kD autoantigen from human placenta...

Reactive antigenic epitopes on presumed autoantigens of biologic interest have been examined by many researchers. The central third complementarity-determining region (CDR3) residues of a human monoclonal anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 regions. Examination of four other...

Based on the complete nucleic acid sequence of human estrogen dependent oviductal protein and deduced amino acid sequence, potential antigenic site of the protein was identified. Two oligonucleotide primers were designed to specifically amplify the region which includes this antigenic site. With the expectation that the human, and monkey oviductins would have high nucleotide sequence homology, ...

We describe a multiple strain Susceptible Infected Recovered deterministic model for the spread of an influenza subtype within a population. The model incorporates appearance of new strains due to antigenic drift, and partial immunity to reinfection with related circulating strains. It also includes optional seasonal forcing of the transmission rate of the virus, which allows for comparison bet...