Among various food sources, milk proteins remain the major vector for functional peptides endowed with several biological activities. Particularly, proteolytic activity of lactic acid bacteria during fermentation has been one most followed strategies to produce bioactive peptides. In present study, exploration starter cultures, at different times, was firstly investigated by reversed phase-high...

This work reports the production of a novel alkaline protease from the fungus Aspergillus niger. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and DEAE-sepharose ion exchange chromatography with a 13.9-fold increase in specific activity. The molecular weight of the enzyme was estimated to be 32 kDa on SD...

Marine biofunctional peptides are protein molecules that promote health by blocking certain harmful chemical pathways in the human body, such as the laying down of cholesterol in blood vessels. Marine bivalves are considered one such rich source of these peptides and bioactive compounds. These, bioactive peptides possess various biological activities such as antibacterial, antioxidant, anticoag...

In recent years, much attention has been paid to the existence of peptides with biological activities and proteins derived from foods that might have beneficial effects for humans. Fishes are rich sources of structurally diverse bioactive compounds. The importance of fish as a source of novel bioactive substances is growing rapidly. Marine fish is a major source of high-quality peptides, protei...

Glutathione, the most abundant intracellular antioxidant, protects cells against reactive oxygen species induced oxidative stress and regulates intracellular redox status. We found that rice peptides increased intracellular glutathione levels in human hepatoblastoma HepG2 cells. Acetaminophen is a commonly used analgesic. However, an overdose of acetaminophen causes severe hepatotoxicity via de...

RATIONALE Apolipoprotein (apoA)-I mimetic peptides are a promising type of anti-atherosclerosis therapy, but how the structural features of these peptides relate to the multiple antiatherogenic functions of HDL is poorly understood. OBJECTIVE To establish structure/function relationships of apoA-I mimetic peptides with their antiatherogenic functions. METHODS AND RESULTS Twenty-two bihelica...

Recent studies have demonstrated that wheat peptides protected rats against non-steroidal anti-inflammatory drugs-induced small intestinal epithelial cells damage, but the mechanism of action is unclear. In the present study, an indomethacin-induced oxidative stress model was used to investigate the effect of wheat peptides on the nuclear factor-κB(NF-κB)-inducible nitric oxide synthase-nitric ...

The antimicrobial peptide database (APD, http://aps.unmc.edu/AP/) is an original database initially online in 2003. The APD2 (2009 version) has been regularly updated and further expanded into the APD3. This database currently focuses on natural antimicrobial peptides (AMPs) with defined sequence and activity. It includes a total of 2619 AMPs with 261 bacteriocins from bacteria, 4 AMPs from arc...

Antioxidant peptides obtained from snakehead (Channa argus) soup (SHS) after simulated gastrointestinal (GI) digestion were separated, identified and characterized. Results showed that the fraction with MW < 3 kDa had highest antioxidant capacity. Four novel RP-HPLC UPLC-MS/MS. PGMLGGSPPGLLGGSPP SDGSNIHFPN DPPH radical scavenging activity (IC50 = 1.39 mM) Fe2+ chelating ability 4.60 mM), respec...

background: antimicrobial peptides with relative length (2-100 amino acids) and positive charge (pure charge (+9)-(+2)) are amphiphilic that isolated from a wide range of animals. recently, these peptides have been known as a part of innate immune response. nowadays, more than 500 antimicrobial peptides from animals have been reported. objectives: the aim of this study was to evaluate the anti-...