The goal of precision therapy is to efficiently treat cancer without side effects. Aptamers are a class of small ligands composed of single-stranded oligonucleotides that bind to their targets with high affinity and specificity. In this study, we identified an ssDNA aptamer specifically targeting Maver-1 lymphoma cells with high binding affinity (Kd = 70±8 pmol/L). Interestingly, cellular cycle...

Aptamers have recently emerged as an excellent alternative to antibodies because of their inherent stability and ease of modification. In this paper, we describe the development of an aptamer-based surface for capture of cells expressing CD4 antigen. The glass or silicon surfaces were modified with amine-terminated silanes and then modified with thiolated RNA aptamer against CD4. Modification o...

Aptamer microarrays are a promising high-throughput method for ultrasensitive detection of multiple analytes, but although much is known about the optimal synthesis of oligonucleotide microarrays used in hybridization-based genomics applications, the bioaffinity interactions between aptamers and their targets is qualitatively different and requires significant changes to synthesis parameters. F...

Riboswitches are non-coding RNAs that control gene expression by sensing small molecules through changes in secondary structure. While secondary structure and ligand interactions are thought to control switching, the exact mechanism of control is unknown. Using a novel two-piece assay that competes the anti-terminator against the aptamer, we directly monitor the process of switching. We find th...

An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.

Portable, easy-to-use and cost-effective sensing devices are desirable in healthcare, environmental monitoring and food safety. Herein we employ polyelectrolyte-aptamer (PE-aptamer) multilayered films that exhibit target-responsive permeability for colorimetric and electrochemical sensing. We present the quantitative detection of an exemplary small molecule, quinine, and address the potential f...

Using SELEX (systematic evolution of ligands by exponential enrichment), we serendipitously discovered a ssDNA aptamer that binds selectively to the anti-FLAG M2 antibody. The aptamer consisted of two motifs (CCTTA and TGTCTWCC) separated by 2-3 bases, and the elimination of one or the other motif abrogated binding. The DNA aptamer and FLAG peptide competed for binding to the antigen-binding po...

A facile and sensitive aptamer-based protocol has been developed for protein assay on microfluidic platform with fluorescence detection using an off-chip microarray scanner. Aptamer-functionalized magnetic beads were used to capture thrombin that binds to a second aptamer fluorescently labeled by Cy3. Experimental conditions, such as incubation time and temperature, washing time, interfering pr...

Aptamer is widely used in the detection field as a new type of probe due to their advantages easy synthesis and modification. Due traditional aptamer screening method, aptamers that are screened by SELEX technology generally have redundant sequences, which affect binding affinity sensitivity. In this study, structural transformation strategy for rationally shortening was established analyzing s...

a label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (ma) by the immobilization of thiolated self-assembled dna sequences on a gold nanoparticles-chitosan modified electrode is constructed. when ma was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...