ADP-ribosylation of the 1:1 (G-A) and 1:2 (G-A-A) gelsolin-actin complexes by Clostridium perfringens iota toxin and Clostridium botulinum C2 toxin was studied. Iota toxin ADP-ribosylated actin in the G-A complex from human platelets as effectively as skeletal muscle actin. The Km for NAD (4 microM) was identical for both substrates. C2 toxin ADP-ribosylated actin in the G-A complex with lower ...

Skeletal muscle androgen receptor (AR) expression at the onset of functional overload (OV) has not been well described. It is also not known if overload and/or anabolic steroid differentially regulate AR expression. The purpose of this study was to examine AR gene expression at the onset of functional OV in rat plantaris muscle with and without nandrolone decanoate (ND) administration. The func...

The endogenous adrenergic agonists norepinephrine (NE) and epinephrine regulate vascular tone by stimulating alpha(1)-adrenergic receptors (ARs) on smooth muscle cells to cause contraction. In addition, alpha(1)-ARs also couple to growth factor pathways, through stimulation of mitogen-activated protein kinases (MAPKs). MAPKs are a family of serine-threonine kinases that include extracellular si...

Background—The cAMP-elevating A 2b adenosine receptor (A 2b AR) controls inflammation via its expression in bone marrow cells. Methods and Results—Atherosclerosis induced by a high-fat diet in apolipoprotein E– deficient mice was more pronounced in the absence of the A 2b AR. Bone marrow transplantation experiments indicated that A 2b AR bone marrow cell signals alone were not sufficient to eli...

BACKGROUND The cAMP-elevating A(2b) adenosine receptor (A(2b)AR) controls inflammation via its expression in bone marrow cells. METHODS AND RESULTS Atherosclerosis induced by a high-fat diet in apolipoprotein E-deficient mice was more pronounced in the absence of the A(2b)AR. Bone marrow transplantation experiments indicated that A(2b)AR bone marrow cell signals alone were not sufficient to e...

background: interleukin-17 (il-17), as a potent proinflammatory cytokine, has a critical role in post liver transplant outcomes. however, there is not much information about the effects of il-17 cytokine on acute liver rejection. objective: to evaluate the role of il-17 in post-liver transplant acute rejection. methods: ninety seven adult liver transplant patients who enrolled in this cross sec...

Parent bodies of meteorites have experienced a variety of thermal events caused by early internal metamorphism and impact heating. Among radiometric chronometers, the 39 Ar-40 Ar technique is particularly sensitive to such thermal events. For over two decades the JSC laboratory has acquired 39 Ar-40 Ar data on a wide variety of meteorite types. The purpose here is to summarize some JSC data (an...

BACKGROUND Acute rejection (AR) of kidney transplants is associated with the loss of endothelial integrity, microvascular rarefaction and, ultimately, graft dysfunction. Circulating angiogenic microRNAs (miRNAs) may serve as markers for microvascular injury. Here, we investigated the short- and long-term effects of AR after kidney transplantation on systemic vascular injury and the associated c...

Background—In the heart, striking functional differences exist after stimulation of the 1and 2-adrenergic receptor (AR) subtypes. These may be linked to differences in metabolic response during 1and 2-AR stimulation. Methods and Results—The relation between work and metabolism was examined during selective 1and 2-AR stimulation ( 1 and 2 groups, respectively) in the isolated perfused rat heart....