VDI type-1 rig and JIS rig are the two major testing rigs for cleanable fabric filters. We measured the filter cleaning performance using these rigs and the results were compared in order to characterize the two testing methods. The filter performance tests showed that the filter cleaning efficiency measured with VDI type-1 rig is higher than that with JIS rig. During pulse jet cleaning, JIS ri...

Animal cells use a group of innate immune sensors to detect viral invasion. The RIG-I-like receptor (RLR) family of RNA helicases is largely responsible for detecting replicating viral RNAs in the cytosol of most, if not all, cells infected with RNA viruses (Rehwinkel & Reis e Sousa, 2010). Two RLRs, RIG-I and MDA5, recognize different types of viral RNA, and thus launch antiviral immune respon...

RIG-I is a well-studied sensor of viral RNA that plays a key role in innate immunity. p97 regulates a variety of cellular events such as protein quality control, membrane reassembly, DNA repair, and the cell cycle. Here, we report a new role for p97 with Npl4-Ufd1 as its cofactor in reducing antiviral innate immune responses by facilitating proteasomal degradation of RIG-I. The p97 complex is a...

Sensing of viral RNA by the cytosolic receptors RIG-I and melanoma differentiation-associated gene 5 (MDA5) leads to innate antiviral response. How RIG-I and MDA5 are dynamically regulated in innate antiviral response is not well understood. Here, we show that TRIM38 positively regulates MDA5- and RIG-I-mediated induction of downstream genes and acts as a SUMO E3 ligase for their dynamic sumoyl...

The relative roles of the endosomal TLR3/7/8 versus the intracellular RNA helicases RIG-I and MDA5 in viral infection is much debated. We investigated the roles of each pattern recognition receptor in rhinovirus infection using primary bronchial epithelial cells. TLR3 was constitutively expressed; however, RIG-I and MDA5 were inducible by 8-12 h following rhinovirus infection. Bronchial epithel...

Retinoic acid-inducible gene I (RIG-I) initiates a rapid innate immune response upon detection and binding to viral ribonucleic acid (RNA). This signal activation occurs only when pathogenic RNA is identified, despite the ability of RIG-I to bind endogenous RNA while surveying the cytoplasm. Here we show that ATP binding and hydrolysis by RIG-I play a key role in the identification of viral tar...

the ability to perform rolling contact fatigue (rcf) experiments in wheel–rail material is provided by a new small–scale test rig, manifesting the actual dynamic behaviour of the railway system. in this paper, a multi-body dynamics (mbd) model is proposed, simulating the vibration behaviour of the prescribed rig. the new testing facility is modelled using a three-dimensional model of the vehicl...

1. A full literature review was completed. 2. A dynamic model of the system was completed and simulation results were used to design and construct the test rig. 3. A fully operational test rig (mini backhoe arm) has been installed and instrumented in the ABE Fluid Power laboratory at Purdue University. 4. Data has been collected on the test rig while operating the system in various modes. 5. Re...

UNLABELLED Hepatitis E virus (HEV) causes both the endemic and epidemic spread of acute hepatitis in many parts of the world. HEV open reading frame 3 (ORF3) encodes a 13-kDa multifunctional protein (vp13) that is essential for HEV infection of animals. The exact role of vp13 in HEV infection remains unclear. In this study, vp13 was found to enhance interferon (IFN) production induced by poly(I...