PURPOSE Experiments were conducted to examine how elevation of calcium in the lens impairs the lens sodium pump. METHODS Rabbit lenses were incubated in the presence or absence of calcium ionophore A23187. 86Rb uptake by the intact lens was measured as an index of sodium pump activity. Na,K-ATPase (ouabain-sensitive adenosine triphosphate [ATP] hydrolysis) activity was determined in membrane ...

The present study investigated the effect of rutin on high glucose-induced actin, α2, smooth muscle, aorta (ACTA2) and p38 protein expression in diabetic nephropathy (DN). Human mesangial cells were divided into a control group, high glucose-induced mesangial cell group, high glucose + captopril group, and high glucose + rutin group (low, middle and high doses of rutin). Cell viability, adenosi...

In insulin-producing cells of the RINmSF line, the nonmetabolized analogue of i.-leucine, 2-aminobicyclo|2,2,1 |heptane-2-carboxylic acid de creases <), consumption, lowers ATP content, and inhibits insulin release despite stimulation of both Ml, production and "(<)., output from cells prelabeled with L-|i/-uC]glutamine. The metabolic and secretory effects of 2-aminobicyclo|2,2,l|heptane-2-carb...

Similar to ischemic preconditioning, diazoxide was documented to elicit beneficial bioenergetic consequences linked to cardioprotection. Inhibition of ATPase activity of mitochondrial F(0)F(1) ATP synthase may have a role in such effect and may involve the natural inhibitor protein IF(1). We recently documented, using purified enzyme and isolated mitochondrial membranes from beef heart, that di...

OBJECTIVES The purpose of this study was to investigate the relationship between hepatic triglyceride content and both myocardial function and metabolism in type 2 diabetes mellitus (T2DM). BACKGROUND Heart disease is the leading cause of mortality in T2DM. Central obesity and hepatic steatosis, both hallmark abnormalities in T2DM, have been related to increased risk of heart disease. METHO...

Triggering of the permeability transition pore (PTP) in isolated mitochondria causes release of matrix Ca2+, ions, and metabolites, and it has been proposed that the PTP mediates mitochondrial Ca2+ release in intact cells. To study the role of the PTP in mitochondrial energy metabolism, the mitochondrial content of Ca2+, Mg2+, ATP, and ADP was determined in hormonally stimulated rat livers perf...

Shao-Yin-Ren Shi-Quang-Da-Bu-Tang (SDT) has been used traditionally to improve the systemic blood circulation and biological energy production in the body. The object of this study is to determine the effect of SDT extract on the decline of cerebral adenosine triphosphate (ATP) and choline content associated with learning and memory impairments in senescence-accelerated mice prone 8 (SAM P8). T...

The effect of whole-body irradiation on rat thymus, 3 differ ent mouse lymphosarcomas, and a mouse myeloid leukemia were studied. In thymus, myeloid leukemia, and C57BL-lymphosarcoma, the inhibition of nuclear adenosine-5'-triphosphate (ATP) synthesis and mitochondrial oxidative phosphorylation is demonstrated within a few hr after irradiation. The inhibition of nuclear ATP synthesis precedes t...

UNLABELLED ATP is a key molecule of cell physiology, but despite its importance, there are currently no methods for monitoring single-cell ATP fluctuations in live bacteria. This is a major obstacle in studies of bacterial energy metabolism, because there is a growing awareness that bacteria respond to stressors such as antibiotics in a highly individualistic manner. Here, we present a method f...

The effect of adenosine triphosphate (ATP) on the release of acetylcholine (ACh) from presynaptic nerve terminals was investigated in bullfrog sympathetic ganglia. ATP decreased the amplitude and quantal content of fast excitatory postsynaptic potentials (fast e.p.s.p.s) calculated from the variance of fast e.p.s.p. amplitude in a low Ca2+-high Mg2+ Ringer solution. The frequency of miniature e...