A series of 10 patients at Armed Forces Institute of Ophthalmology, Rawalpindi, from September 2009 to August 2010 is reported. Follow-up of patients were done till February 2012. Patients with persistent corneal epithelial defects (PED) of minimum 2 weeks duration, in whom conventional therapy had failed, were included in the study. They were prescribed 100% autologous serum eye drops on hourl...

CD4+ T cells are sensitive to peripheral changes of cytokine levels and metabolic substrates such as glucose lactate. This study aimed analyze whether factors released after exercise alter parameters human cell metabolism, specifically glycolysis oxidative phosphorylation. We used primary activated in the presence autologous serum, which was collected before (CO) a 30-min intervention (EX). In ...

I Chronic idiopathic urticaria (CIU) is the most frequent form of urticaria, accounting for 70-80% of the cases (1). CIU is defined as a 6-week or longer history of urticaria in the absence of detectable physical, allergic, vasculitic, infectious or drug-induced causes (2). The presence of autoantibodies directed toward IgE or to the high-affinity IgE Fc receptor, detected by intradermal test w...

The response of peripheral blood lymphocytes (PBL) to autologous synovial fluid lymphocytes (SFL) from patients with rheumatoid arthritis and Reiter's syndrome was investigated in an autologous mixed lymphocyte reaction (AMLR). SFL were found to be poor responders but strong stimulators of autologous and allogeneic PBL compared with autologous PBL. The plastic-adherent (macrophage) cells from t...

Introduction. Coagulation and blood clot formation in bone defects is sometimes followed by retraction of a blood clot and serum extrusion, thus producing peripheral serum-filled spaces between bony wall and coagulum. This can result in a higher incidence of postoperative complications. Stabilization of blood coagulum, which enables successful primary healing, may be accomplished by autotranspl...

The in vitro lymphoproliferative responses to herpes simplex virus (HSV) antigens were followed in rabbits with herpesvirus infection of the cornea. The proliferative cellular responses occurred early in infection and were demonstrated by lymphoid cells from the local lymph nodes at day 5, the peripheral blood at day 11, and the spleen after day 14. The presence of autologous serum antibodies s...

Sera from 151 patients with a variety of cancers were screened for antibody-like activity against lipoproteins. Eighteen % of the sera exhibited activity against autologous and homologous high-density lipoproteins and 3% exhibited activity autologous and homologous low-density lipoprotiens. Antibody-like binding was proven by its restriction to the Fab fragment of IgG. The reactive part of the...

The uptake of tritiated thymidine by lymphocytes from 100 cancer patients and 31 normal subjects was tested in the presence and absence of phytohaemagglutinin (PHA) in a medium containing autologous or homologous serum. Significant differences were found between normal subjects and cancer patients in both the time course and degree of uptake. Serum from normal individuals restored the response ...

Induction of antitumor immunity using autologous tumor proteins is an attractive approach to cancer therapy. However, better methods and stimulants to present these autologous proteins back to the immune system are needed. Here, we identify streptavidin as a novel carrier protein and stimulant, and test the efficacy of both syngeneic (rat) and autologous vaccines (dogs) using streptavidin in co...