We have demonstrated, with an in vitro model, that Staphylococcus epidermidis is able to colonise intraocular lenses. Adherent organisms were quantitated by light microscopy, scanning electron microscopy, and viable counting. Bacterial adherence was associated with production of a polysaccharide glycocalyx. Organisms which were attached to the lenses were resistant to apparently bactericidal co...

PURPOSE Management of endophthalmitis typically includes antibiotic combinations to arrest bacterial growth and antiinflammatory agents to limit inflammatory damage to sensitive tissues. Little research has been reported that systematically evaluates the contribution of each therapeutic component for treating infections caused by organisms of varying virulence. The authors determined the relati...

Intravitreal drug administration is the treatment of choice for bacterial endophthalmitis, but improved knowledge of vitreal pharmacokinetics is essential for the development of optimal antibiotic regimes. We used our recently developed sampling device to estimate vitreal gentamicin concentrations for up to 30 hr after an intravitreal bolus injection of gentamicin. The device is based on the pr...

PURPOSE In a study complementing a previous multicenter randomized clinical trial on prophylactic injection of intraocular antibiotics during primary repair of penetrating eye injuries (PEIs), we sought to determine whether needle entrance and injection of balanced salt solution (BSS), per se, could increase the rate of acute post-traumatic bacterial endophthalmitis (APBE). METHODS Patients r...

Staphylococcus (S.) aureus is a common causative agent of bacterial endophthalmitis, a vision threatening complication of eye surgeries. The relative contribution of S. aureus virulence factors in the pathogenesis of endophthalmitis remains unclear. Here, we comprehensively analyzed the development of intraocular inflammation, vascular permeability, and the loss of retinal function in C57BL/6 m...

AIM To assess the incidence of anterior chamber bacterial contamination during phacoemulsification surgery using an automated microbial detection system (BacT/Alert). METHODS Sixty-nine eyes of 60 patients who had uneventful phacoemulsification surgery, enrolled in this prospective study. No prophylactic topical or systemic antibiotics were used before surgery. After antisepsis with povidone-...

Preventing PostoPerative infection Postoperative endophthalmitis is a rare but potentially devastating complication of cataract surgery. The incidence of postoperative endophthalmitis is small and most recent studies have found that in the United States, the rate is approximately 0.07%. With an ever-aging population, the number of patients requiring cataract surgery is growing each year. Theref...

Graph or image title Endophthalmitis is a rare, but devastating outcome after intraocular surgery, trauma, systemic or local infection. It is characterized by inflammation involving the vitreous cavity and the anterior chamber of the eye. Once suspected, patients are subjected to ocular tissue biopsies, with microbial cultures, antibiotics delivered in multiple routes, and sometimes surgery to ...

Purpose: Since standard microbiological methods (use of solid media) have low sensitivity to detect aetiological agents in vitreous aspirates in infectious endophthalmitis, a comparative study was undertaken to determine sensitivity of BACTEC Peds Plus F broth for isolation of these aetiological agentsMethods: Four hundred and eighty three consecutive vitreous aspirates from clinically diagnose...

BACKGROUND Endogenous bacterial endophthalmitis is an infrequently reported entity. Although Roseomonas mucosa has been reported to cause systemic infections in immunosuppressed individuals, ocular infection due to Roseomonas has been rarely reported in literature previously. FINDINGS A 74-year-old diabetic was diagnosed to have Klebsiella urinary tract infection and septicemia following whic...