Introduction Characterization of the glycosylation profile of a recombinant protein product is an important part of defining product quality in the bioproduction industry. Development of a protein with desired characteristics would require the capacity to modify and target specific glycosylation patterns as well as an understanding of the implications of changes to these glycosylation profiles....

The overcoming of catabolite repression, in bioinsecticides production by sporeless Bacillus thuringiensis strain S22 was investigated into fully controlled 3 L fermenter, using glucose based medium. When applying adequate oxygen profile throughout the fermentation period (75% oxygen saturation), it was possible to partially overcome the catabolite repression, normally occurring at high initial...

Nonoverlapping batch means (NOLBM) is a a we11-known approach For For estimating the variance of the sample mean. In this paper we consider an overlapping batch means (OLBM) estimator that, based on the same assumptions and batch size as as NOLBM, has has essentially the same mean and only 2/3 the asymptotic variance of NOLBM. Confidence interval procedures for the nlean based on NOLBM and OLBM...

Foster, McDaniel, Woodruff, and Stokes (1945) recently described the formation of conidia by molds of the genus Penicillium during submerged growth in liquid culture media. Controlling or associated factors for this phenomenon were described as the presence of a rather high calcium-ion concentration and, in general, the development of considerably less mycelium before spore formation than is fo...

The Distributed Batch Controller (DBC) supports scientiic batch data processing. The DBC distributes batch jobs to one or more pools of workstations and monitors and controls their execution. The pools themselves may be geographically distributed, and need not be dedicated to processing batch jobs. We describe the use of the DBC in a large scientiic data processing application, namely the gener...

A dynamic flux balance model based on a genome-scale metabolic network reconstruction is developed for in silico analysis of Saccharomyces cerevisiae metabolism and ethanol production in fed-batch culture. Metabolic engineering strategies previously identified for their enhanced steady-state biomass and/or ethanol yields are evaluated for fed-batch performance in glucose and glucose/xylose medi...

M athematical model–based simulations of actual bioreactor runs suggest how process variables such as substrate and product concentrations change and how nutrient feeding should be “tuned” with respect to time, pattern, concentration, and composition to elicit a desired response. Insights gained from modeling can guide us in the adjustment of a process, reducing the number of characterization r...