Cellulase (beta-1,4-glucan-glucanohydrolase EC 3.2.1.4) activity increased during abscission and was localized in the cell separation layer of Phaseolus vulgaris L. cv. Red Kidney (bean), Gossypium hirsutum L. cv. Acala 4-42 (Cotton) and Coleus blumei Benth. Princeton strain (Coleus) abscission zone explants. Cellulase activity was optimum at pH 7, was reduced by one-half after heating to 55 de...

This experiment was performed to evaluate the effects of different types and concentrations of carbohydrate source on callus fresh weight, number of somatic embryos per embryonic explant and percentage of globular embryos developing into cotyledonary embryos from the leaf explants of three strawberry cultivars (Kurdistan, Paros and Camarosa). MS medium containing 1.0 mg/l 2,4-dichlorophenoxyace...

and color of the treated area which has been suggeste(l to act as a metabolic siiik (6). The stimulation of both RNA and l)rotein synthesis b) kinetic in tobacco (8) aiid Xmanthinmn (7) leaves has been reported, lanl MIcCalla, 'Moore and Osborne (5) using another kinin, the benzyladenine-C14, have obtained tentative evideence that label was incorporated into RNA although at a very low rate. Wol...

The accumulation of chlorophyll, production of two specific lamellar chlorophyll-protein complexes, onset of O(2) evolution, and detection of P700 were examined in intact Jack bean (Canavalia ensiformis [L.] D.C.) leaves treated with 10(-5)m kinetin or benzyladenine and allowed to green under low (30-35%) and high (80-85%) relative humidity. In contrast to reports of the promotion of chlorophyl...

An efficient and highly reproducible protocol for micropropagation of bird eye chilli Capsicum frutescens was attempted. Murashige and Skoog (MS) medium containing 0.5-3.0 mgl(-1) of 6-benzyladenine (BA), 2-isopentenyl adenine (2iP), kinetin and 0.5-2.0 mg l(-1) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) along with 1 gl(-1) activated charcoal (AC) were used for shoot regeneratio...

"Aging" of excised cotyledons plus the top part of the hypocotyl of Amaranthus tricolor seedlings was carried out by washing in distilled H(2)O for varying periods. This led to increased betacyanin accumulation during the subsequent 24-hour induction period in the presence of tyrosine and Na(+) + K(+) phosphate. Endogenous accumulation as well as that dependent on added benzyladenine and on add...

Random genetic changes generated during in vitro culture are not desirable for plant micropropagation and genetic transformation. RAPD markers were used to detect the variation in leaf disc callus cultures of Jatropha curcas, maintained in Murashige and Skoog (MS) medium with different auxin and cytokinin combinations. In total 41 scorable bands were produced with 11 primers. Out of 41 bands, 3...

A method of in vitro clonal propagation of cowslip Primula veris L. (Primulaceae) is reported. This is a species of medicinal importance, protected by law in Poland. MS medium Murashige and Skoog (1962) with 6 benzyladenine BA (4.44 μM) and 2,4-dichlorophenoxyacetic acid 2,4-D (1.13 μM) was found to be optimal for in vitro cowslip propagation from shoot tips under the conditions of culture. Roo...

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was...

Tendrils produced from shoot tips of grapevine (Vitis vinifera L.) cultured in vitro on Nitsch's medium developed into inflorescences when 5 to 10 mum benzyladenine (BA) or 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (PBA) were applied directly to the tendril tips. Inflorescences did not form on tendrils if the cytokinins were supplied in the agar. Tendrils cultured in agitated liquid med...