Biochemical investigations with isolated blowfly flight muscle mitochondria show that uncoupling of oxidation and phosphorylation increases with increasing age of the adult fly. Of the two principal substrates used (i.e. a-glycerophosphate and pyruvate), uncoupling is most marked when a-glycerophosphate is used as substrate. Although age-related changes in the structure of the thoracic flight m...

The phosphatidyl glycerophosphate B phosphatase of Escherichia coli has a multiple substrate specificity and a peculiar dual subcellular localization in the envelope. Its phosphatidyl glycerophosphate phosphatase activity is higher in the cytoplasmic membrane, while phosphatidic acid and lysophosphatidic acid phosphatase activities are higher in the outer membrane. The DNA sequencing of the pgp...

A biochemical and cytochemical study has been made of the distribution of ATPase in mature and differentiating phloem cells of Nicotiana tabacum and of the substrate specificity and effects of fixation on enzyme activity. Homogenates of unfixed leaf midveins and midveins fixed in formaldehyde-glutaraldehyde were assayed for enzyme activity by determining the amount of P(i), liberated per millig...

A method for detecting possible structural genes in D. melanogaster based on gene dosage dependency is presented. By making thirty crosses between Y-autosome translocations, and an attached-4 cross, it is possible to produce large duplications (approximately 150 salivary gland chromosome bands in length) for every autosomal region with the exception of 83DE. The usefulness of the technique was ...

The combination of an antifungal agent and drug carrier with adjunctive antimicrobial properties represents novel strategy of complex therapy in pharmaceutical technology. The goal of this study was to investigate the unmodified and ion cross-linked chitosan's influence on anti-Candida activity of clotrimazole used as a model drug in hydrogels. It was particularly crucial to explore whether the...

F OR A NUMBER OF YEARS this laboratory has been interested in the phosphatase activity of isolated human leukocytes and the marked lability of leukocyte alkaline phosphatase in disease.1 3 Leukocyte alkaline phosphatase has been demonstrated to be essentially limited to the granulocytic cells and to be characteristically low in chronic myelocytic leukemia. Elevated values are noted in polycythe...