Clostridial and spinach ferredoxins, reduced enzymatically by the action of ferredoxin-TPN+ oxidoreductase, have been shown to carry out the univalent reduction of oxygen. The superoxide radicals, so generated, were detected by their ability to cause the oxidation of epinephrine to adrenochrome. Superoxide dismutase prevented the production of adrenochrome in these reaction mixtures, while havi...