host innate immunity can affect the clinical outcomes of helicobacter pylori infection, including gastritis, gastric ulcer, gastric adenocarcinoma, and malt lymphoma. nucleotide binding oligomerization domain (nod)-1 and -2 are two molecules of innate immunity which are involved in the host defense against h. pylori . this study aimed to evaluate the effect of the expression level of nod1 and n...

ba ckground: obesity is a consequence of the excessive accumulation of fat in adipose tissue which can result in significant morbidity and mortality. obesity is a major health problem in iran.  objective: the aim of this study was to investigate the  effects of  epa consumption on bmi  and  fasting blood sugar by fabp2 genotypes and pparα (leu162val, and g/c intron polymorphism) genotypes.   m ...

urease was immobilized on platinum electrode both by chemical binding and electropolymerization.the conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 m and linear dynamic range from 4.9×10-5 to 5.8×10-3 m for urea concentration when the enzyme is covalently immobilized on pt electrodes. conductometric transducers respond to the changes in ionic strength thereby le...

the binding behavior of a competitive inhibitor, n-phenylbenzhydroxamic acid (bha) against horseradish peroxidase (hrp) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. the dissociation constants of the complexes of hrp-bha, hrp-donor and hrp-bha-azide were estimated at specified conditions by difference spectroscopy. the binding site ...

neutral bidentate n-o type complexe of vo(ii) has been synthesized through condensation of dpenicillamine,(d-h2pen), with bis(acetylacetonate)- oxovanadium(iv),[vo(acac)2], at neutralmedium . the established schiff base(no type) complex was characterized on the basis of ir andmass spectroscopy techniques.besides,d-h2pen binding behavior to[vo(acac)2] has been studiedin thermodynamic viewpoint u...

the surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. in this study, conformational changes of glucose oxidase (gox) in the mercaptopurine: gnps and 11-mercaptoundecanoic acid: gnps as a hydrophobic and a hydrophilic gnps surface was investigated by various spectroscopic techniques, including: uv-vis absorption, f...

more recently medical chemistry research has been focused on proteins that drive and controlcell cycle progression. among them, the cyclin dependent kinases (cdk’s) are a group ofserine/threonine kinases, which rule the transition between successive stages of the cell cycle. theactivity of cdk’s is regulated by multiple mechanisms, including binding to cyclins, which is a broadclass of positive...

a study was made on the interaction between histon h1 and sodium n-dodecyl sulphate (sds) in the presence of sodium arsenate inside a phosphate buffer of ph 6.4, using spectroscopy and equilibrium dialysis at 27 °c. the binding data has been used to obtain the gibbs free energy in terms of a theoretical model based on the wyman binding potential. the binding data hs been analysed in terms of hi...

background: tubulin protein being the fundamental unit of microtubules is actively involved in cell division thus making them a potential anti-cancer drug target. in spite of many reported drugs against tubulin, few of them have started developing resistance in human β-tubulin due to amino acid substitutions. methods: in this study we generated three mutants (f270v, a364t and q292e) using model...

glucose 6- phosphate dehydrogenase from streptomyces aureofaciens was purified andinactivated by pyridoxal 5′-phosphate (plp). the inactivation was a pseudo-first order and time-dependentreaction. complete inactivation was achieved at 0.2mm plp within 16 minutes. the type of inhibition wascompetitive with respect to glucose 6- phosphate. spectral characteristics of plp-enzyme complexcorresponde...