We present the coupling of atomic force microscopy (AFM) and nuclear magnetic resonance (NMR) technologies to enable topographical, mechanical, and chemical profiling of biological samples. Here, we fabricate and perform proof-of-concept testing of radiofrequency planar microcoils on commercial AFM cantilevers. The sensitive region of the coil was estimated to cover an approximate volume of 19....

Although classical genetic and biochemical approaches have identified hundreds of proteins that function in the dynamic remodeling of cell shape in response to upstream signals, there is currently little systems-level understanding of the organization and composition of signaling networks that regulate cell morphology. We have developed quantitative morphological profiling methods to systematic...

Numerous studies on complications associated with eating disorders have been conducted worldwide. However such studies are limited in the Indian scenario. Hence, we attempted to analyse the presence of oxidative stress along with total lipid profiling of students with eating distress in Mysore, South India. A biochemical test panel was conducted using serum samples of controls and subjects. Res...

Cancer cells possess fundamentally altered metabolism that provides a foundation to support tumorigenicity and malignancy. Our understanding of the biochemical underpinnings of cancer has benefited from the integrated utilization of large-scale profiling platforms (e.g., genomics, proteomics, and metabolomics), which, together, can provide a global assessment of how enzymes and their parent met...

Whispering gallery mode resonances in liquid droplets and microspheres have attracted considerable attention due to their potential uses in a range of sensing and technological applications. We describe a whispering gallery mode sensor in which standard optical fibre is used as the whispering gallery mode resonator. The sensor is characterised in terms of the response of the whispering gallery ...

We recently described a fluorescence polarization platform for competitive activity-based protein profiling (fluopol-ABPP) that enables high-throughput inhibitor screening for enzymes with poorly characterized biochemical activity. Here, we report the discovery of a class of oxime ester inhibitors for the unannotated serine hydrolase RBBP9 from a full-deck (200,000+ compound) fluopol-ABPP scree...

The success of conception in buffalo is greatly dependent on precise estrus detection and time of artificial insemination (AI). Various visual, behavioral, biochemical and gyneco-clinical parameters have been tracked closely and a cost-effective combinatorial model has been developed to detect estrus in buffaloes. Pheromones play pivotal roles in reproduction and behavior of mammals. Urine, an ...

The need for clean groundwater resources to have sustainable development in a country is undoubted. Due to the importance and high quality of karstic waters in supplying water in Iran especially in Shahrood city, it is attempted in this research work to recognize and explore karstic waters in southwest of Tepal area, Shahrood. For this purpose, integration of the results obtained from the metho...