The developed duplex real-time PCR method allows the simultaneous detection of traces of potentially allergenic white mustard (Sinapis alba) and celery roots (Apium graveolens var. rapaceum), celery stalks (A. g. var. dulce) and leaf celery (A. g. var. secalinum). The duplex assay does not show any cross-reactivity with 64 different biological species, including various members of the Brassicac...

Sulphur mustard (SM) is a well-known chemical warfare agent. It was first used during World War I and more recently in the Iran/Iraq conflict causing more than 100,000 Iranian casualties. Previous study showed that this gas can raised inflammatory marker but in this current case that suffered from cancer. After examine current case for candidate CABG, we found that Hs-CRP and IL-6 was very low ...

Invitro antimycotic effect of the coconut, mustard, groundnut & soyabeen oils and the seeds of trigonella, ajwoin, mustard and garlic bulbs were mixed in the above oil samples to determine their effect on the spore germination of five pathogenic fungi i.e., Aspergillus niger, A.flavus, Absidia corymbifera, Penicilium nigricans and Candida albicans, isolated from otitic fungal infection of exter...

The exposure of two Iranian victims of the Iran-Iraq conflict (1980-1988) to sulfur mustard was established by immunochemical and mass spectrometric analysis of blood samples taken 22 and 26 days after alleged exposure. One victim suffered from skin injuries compatible with sulfur mustard intoxication but did not have lung injuries; the symptoms of the other victim were only vaguely compatible ...

A technique for inoculation with precise numbers of fungal spores on leaves and stems of plants was developed. The technique consisted of placing 1-μl drops with a fixed number of spores on the surface of leaves and stems, and covering each inoculum drop with a 10-μl drop of low-temperature gelling SeaPlaque agarose to fix the inoculum on the target site. With this technique single zoospores of...

Sulfur mustard or mustard gas has been widely used as a chemical weapon in the first world war and Iraq-Iran war. It causes acute and chronic complications in lungs, eyes and skin. In skin, mustard is toxic, mutagenic, and carcinogenic via alkylation of cellular proteins in enzymes, cell membranes, cytoplasm and particularly cell nucleus as well as in DNA components of the epidermis, dermis and...

Nitrogen mustards are chemical agents that are similar to sulfur mustards, with similar toxicities. The present study describes a case of nitrogen mustard-induced acute respiratory failure and myelosuppression in a 33-year-old man. The patient, who was accidentally exposed to nitrogen mustard hydrochloride in a pharmaceutical factory, exhibited severe inhalation injury and respiratory symptoms....

Restriction fragments of chloroplast (cp)DNA from mustard leaves were cloned in E. coli using pBR 322 as the vehicle. Cloned fragments containing the structural sequences for two polypeptides of 56,000 and 35,000 daltons were selected by a mRNA hybridization-translation procedure. The cloned mustard genes are structurally related to chloroplast genes from maize. They hybridize to the maize mRNA...

Although the survival of surface-attached Chinese hamster cells varies through the growth cycle after either X-irradiation or sulfur mustard treatment, the variations differ. Cells in the latter part of the DNA synthetic phase yield maximum survival after X-rays, while cells in the postsynthetic phase are the most resistant after sulfur mustard. As a result, when asyn chronous cells are treated...