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vijaya shivkumar rajmane department of microbiology, krishna institute of medical sciences university, karad, india; department of microbiology, krishna institute of medical sciences university, karad, india. tel: +91-9860299944, fax: +91-02164243272 shivkumar t rajmane department of orthopedics, institute of medical sciences and research, mayani, india ashok y kshirsagar department of surgery, krishna institute of medical sciences university, karad, india virendra chandrashekhar patil department of medicine, krishna institute of medical sciences university, karad, india