Binding studies of cationic detergents such as cetyltrimethyl ammonium bromide, Cetyl pyridinium bromide and dodecyl trimethyl ammonium bromide with β Lactoglobulin were carried out by equilibrium dialysis, ultraviolet difference and circular dichroism techniques at 25 C. Binding isotherms at pH 5·0, 7·0 and 9·0 show cooperative binding at all concentrations of detergents and the number of avai...

Ethidium bromide-agarose gel electrophoresis is an extensively used procedure for the analysis of DNA; it has been reported to be sensitive to as little as 50 ng of DNA (7). The standard methods for staining gels involve adding 0.5 μg/mL ethidium bromide to both the agarose gel and the running buffer or soaking the gel in a solution of ethidium bromide after electrophoresis has been performed (...