Three alkaline protease producing gram positive, spore forming Streptomyces (J1, J2 and J3) were isolated from alkaline soil (pH range: 9-10) samples collected from Central Soil Salinity Research Institute (C.S.S.R.I), Lucknow. On the basis of halo zone, morphology, texture and microscopic characteristics they were identified as Streptomyces. Further characterization both genetically and phenot...

We evaluated the feasibility of large-scale production of biopharmaceuticals expressed as heterologous polypeptides from the Gram-positive bacterium Streptomyces lividans. As a model protein we used murine tumor necrosis factor alpha (mTNFalpha). mTNFalpha fused C-terminally to the secretory signal peptide of the subtilisin-inhibitor protein from Streptomyces venezuelae. Under appropriate ferme...

Streptomycetes are Gram-positive filamentous soil bacteria that grow by tip extension and branching, forming a network of multinucleoid hyphae. These bacteria also have an elaborate process of morphological differentiation, which involves the formation of an aerial mycelium that eventually undergoes extensive septation into chains of uninucleoid cells that further metamorphose into spores. The ...

MOTIVATION TTA Lynx is a web-based service for finding, assessing and comparing coding sequences that contain TTA codons. This codon is most notable for being a regulatory switch that governs different aspects of the physiology of several GC-rich, Gram-positive bacteria belonging to genus Streptomyces, prolific producers of clinically important natural products. The ever-increasing pace of geno...

Linear plus linear homologous recombination-mediated recombineering (LLHR) is ideal for obtaining natural product biosynthetic gene clusters from pre-digested bacterial genomic DNA in one or two steps of recombineering. The natural product salinomycin has a potent and selective activity against cancer stem cells and is therefore a potential anti-cancer drug. Herein, we separately isolated three...

One of the major obstacles in the discovery of new antibiotics is the absence of a method for rapid recognition of known compounds. The use of microbes resistant to specific groups of antibiotics as a means of eliminating these compounds was proposed in 1946 by EISMAN, et al.l> and more extensively utilized by STAPLEY2>. In a conventional screening program, based on detection of activity agains...

We investigated the role in bacterial infection of a putative ABC transporter, designated ybiT, of Erwinia chrysanthemi AC4150. The deduced sequence of this gene showed amino acid sequence similarity with other putative ABC transporters of gram-negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa, as well as structural similarity with proteins of Streptomyces spp. involved in ...

Six new products of Streptomyces murayamaensis sp. nov. Hata et Ohtani, the producer of the kinamycins, were isolated by silica gel column chromatography. The antibacterial activities of the new products, as well as that of dehydrorabelomycin and murayaquinone, previously isolated products of the same organism, were compared to the kinamycins. Three of the products had antibacterial activities ...