abstract background: determination of transgenic embryos from non transgenic embryos sibling is an important step in producing homozygous transgenic mice. these steps need by pcr or southern blotting followed extraction of dna, but both techniques require skill and consume time. objective: the aim of this study was simulation of high accuracy method using novel enhanced green fluorescent protei...

The effect of multi-step austempering treatments on the microstructure and mechanical properties a novel medium carbon high silicon carbide-free bainitic steel was studied. Five different isothermal treatment processes were selected, including single-step above martensite start temperature (at 350 °C 370 °C, respectively), three kinds two-step routes (370 + 300 250 °C). In comparison with adopt...

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and main cause of cancer-related death worldwide. The available treatment options for HCC include transplant, locoregional therapy (such as ablation, embolization, radiotherapy), systemic treatment. latter encompasses targeted therapy, immunotherapy, angiogenesis inhibitors, alone or in combination. introduction immune check...

Background: Haplotyping is invaluable not only to identify genetic variants underlying a disease or trait, but also to study evolution and population history as well as meiotic and mitotic recombination processes. Current genome-wide haplotyping methods rely on genomic DNA that is extracted from a large number of cells. Thus far random allele drop out and preferential amplification artifacts of...