Halophiles are perceived as an excellent source of novel enzymes possessing inherent ability to function under saline and hypersaline environment conditions. The article covers and puts in perspective the structural and biocatalytic features of α-amylases from halophilic sources. The choice of α-amylase as the target enzyme is based on the fact that this is among the largest selling enzymes. Ol...

This study was designed to investigate the inhibitory effect of Gynura procumbens extract against carbohydrate digesting enzymes and its ability to ameliorate postprandial hyperglycemia in streptozotocin (STZ)-induced diabetic mice. G. procumbens extract showed prominent α-glucosidase and α-amylase inhibitory effects. The half-maximal inhibitory concentration (IC50) of G. procumbens extract aga...

Transtracheal puncture has long been known as a safe, low-cost procedure. However, with the advent of bronchoscopy, it has largely been forgotten. Two researchers have suggested the use of α-amylase activity to diagnose salivary aspiration, but the normal values of this enzyme in tracheobronchial secretions are unknown. We aimed to define the normal values of α-amylase activity in tracheobronch...

When heat shocked, plant cells distribute their energy to make a very different set of proteins than when at normal temperatures. Along with other secretory proteins, α-amylase is particularly affected by heat shock. This protein is responsible for digesting the starchy food stores contained in the endosperm of a barley seed. These starchy nutrients comprise the largest part of the food store t...

Using homogenates of autopsy tissue, we compared three widely available techniques for separating amylase isoenzymes: wheat-germ inhibition (WI), and electrophoresis on cellulose acetate (CA) or agarose (AG). WI separated amylase into two isoforms, CA into seven (three pancreatic and four salivary), and AG into nine (five pancreatic and four salivary). CA and WI had similar isoamylase detection...

Background: Chrysobalanus orbicularis is commonly used as food, the seeds as a spice for the popular pepper soup, and the leaves are traditionally utilized for managing type II diabetes mellitus (T2DM) in the Niger Delta area of Nigeria. Due to the limited scientific evidence on C. orbicularis, this study investigated its antioxidant and inhibitory properties against major enzymes linked to T2D...

This study evaluated the in vitro antioxidative activity of Khaya senegalensis extracts and inhibitory effects of some solvent fractions on α-glucosidase and α-amylase activities. The stem bark, root and leaf samples of the plant were sequentially extracted with ethyl acetate, ethanol and water and then tested for antioxidative activity. Our findings revealed that the ethanolic extract of the r...

BACKGROUND In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities. METHODS Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosiphon stamineus. The structure of this pure compound was determined on the NMR data and the α-gluco...

BACKGROUND Over a 10 year period a decline in the rate of sensitizations to α-amylase (Aspergillus oryzae) was observed in bakers investigated for allergic obstructive airway disease. At the same time, glucoamylase (Aspergillus niger) was identified as the currently the most relevant allergen in sensitizations to enzymes in the baking industry. The aim of the present study was to investigate wh...

The purpose of this study is to discuss the significant role α-amylase enzyme in production and rheological characteristics gluten-free rice dough baked bread. Small deformation large methods were used evaluate effect addition on bread characteristics. baking properties evaluated by specific volume, loss, Texture profile analysis parameters. added microstructure was also evaluated. doughs showe...