BACKGROUND Hematoxylin-eosin (HE) staining of a full-thickness rectal wall fragment is classically used for the diagnosis of Hirschsprung disease (HD). However, this technique requires large fragments for a better diagnosis. Additionally, the histochemical and immunohistochemical methods of staining small fragments of rectal mucosal and submucosal biopsies are not available in all centers. Ther...

Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal cortical dysplasia and int...

Functional imaging studies of the human brain have suggested the involvement of the cingulate gyrus in a wide variety of affective, cognitive, motor, and sensory functions. These studies highlighted the need for detailed anatomic analyses to delineate its many cortical fields more clearly. In the present study, neurofilament protein, and the calcium-binding proteins parvalbumin, calbindin, and ...

This paper aims at presenting a personal experience in teaching the HCI course at the Computer Science Department at the Federal University of Minas Gerais, in Brazil. The course syllabus, how theory and practice are integrated in the course, and the experience to integrate the course work and the Evaluation Competition category available at the Brazilian Symposium of Human Factors in Computing...

Leydig cells have been found to be either unidentifiable or at apparent low numbers during routine histologic examination of bowhead whale testes. Therefore, formalin-fixed, paraffin-embedded testicular tissues from 14 whales were retrospectively examined determine if immunohistochemical staining could aid in identification Sertoli and cells. Multiple intratesticular samples when available. dif...

Citation: Barinka F (2016) Commentary: Lack of functional specialization of neurons in the mouse primary visual cortex that have expressed calretinin. The intracellular protein calretinin (CR) is frequently used as a marker of specific (albeit heterogeneous) cortical interneuronal population (see Cauli et al., 2014; Schwaller, 2014 for recent reviews). A large body of data on connectivity of ca...

Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to...