A study was conducted to determine the seroprevalence of Contagious Caprine Pleuropneumonia (CCPP) in 106 goats Bharatpur Municipality Chitwan, Nepal from July October 2021. questionnaire used potential risk factors. Collected samples were subjected a competitive enzyme-linked immunosorbent assay (cELISA) for detection antibodies. The 4.71% (5/106). Although high among females and below or equa...

Enzootic abortion in ewes (EAE) is routinely diagnosed by the detection of elementary bodies of Chlamydophila abortus in placental smears stained with modified Ziehl-Neelsen’s (MZN) stain. The C. abortus can also be isolated from infected material in McCoy cells or embryonated eggs. Currently, the polymerase chain reaction (PCR) is increasingly being used for the detection and differentiation o...

Caprine placental lactogen (cPL) cDNA was cloned by reverse transcription (RT)-PCR from total RNA of goat placenta. The PCR product encoding for the mature protein was gel purified, ligated to pGEM-T and finally subcloned into a pET8c prokaryotic expression vector. E. coli cells (BL-21) transformed with this vector overexpressed large amounts of cPL upon induction with Isopropyl-1-thio-beta-D-g...

Bovine buffy coat cells infected with the bovine leukemia virus (BLV) induce syncytia formation in human diploid embryonic lung cells as well as in monolayer cell cultures of bovine, simian, ovine, bat, and caprine origin, but not in mouse fibroblast cells, normall rat kidney cells, or RSV-transformed rat cells. Syncytia were not observed in diploid embryonic lung cells inoculated with bovine b...

Follicular atresia mainly results from the apoptosis of granulosa cells (GCs). Whilst our previous investigations examined the role of chi-miR-4110 in regulating ovarian function, the present study detected the role of chi-miR-4110 in GC development. We transfected caprine GCs cultured in vitro with chi-miR-4110 mimics. Results revealed that chi-miR-4110 decreased mRNA and protein levels of Sma...

Heparin is a well-known glycosaminoglycan extracted from porcine intestines. Increased vigilance for transmissible spongiform encephalopathy in animal-derived pharmaceuticals requires methods to prevent the introduction of heparin from ruminants into the supply chain. The sensitivity, specificity, and precision of the quantitative polymerase chain reaction (PCR) make it a superior analytical pl...

The present study was carried out to evaluate the seroprevalence of anti-Toxoplasma antibodies in four caprines breeds that are commonly reared in Cholistan desert, Rajan Pur and Rahim Yar Khan during the period between April 2011 and March 2012. The objective of the present study was to ascertain the infection rates of toxoplasmosis in different caprine breeds so that the disease resistant goa...

This study aims to investigate the effects of follicle stimulating hormone (FSH) and fibroblast growth factor-2 (FGF-2) on the survival and growth of caprine preantral follicles. Ovarian tissues were cultured for 1, 7, 14, 21 or 28 days in medium supplemented with FSH (FSH-2d or FSH-7d, i.e., with replacement of the culture medium every 2 or 7 days, respectively) or FSH+FGF-2 (replacement of th...

Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was re...

Staphylococcus aureus is an important pathogen in domestic ruminants. The main objective of this study was to determine the similarity of epidemiologically unrelated S. aureus isolates from bovine, ovine, and caprine hosts regardless the locus of isolation (nares and udder). By pulsed-field gel electrophoresis, seven major pulsotypes were identified among 153 isolates recovered from 12 differen...