Depending on the duration and severity, psychological tension and physical stress can enhance or suppress the immune system in both humans and animals. Although it is well established that stress alters the release of various hormones and neurotransmitters, the mechanisms by which stress affects immune responses remain elusive. We report here that mice subjected to chronic 12-hour daily physica...

PURPOSE To characterize daunorubicin-induced cell death in cultured human retinal pigment epithelial (RPE) cells and its modulation by CD95 ligand (CD95L). METHODS In situ DNA end labeling and an ELISA for histone-associated DNA fragments were used to assess apoptosis. CD95 and CD95L expression were examined by immunohistochemistry, flow cytometry, immunoblot, and RT-PCR. Cell death was measu...

Blocking angiogenesis is a promising approach in cancer therapy. Natural inhibitors of angiogenesis and derivatives induce receptor-mediated signals, which often result in the endothelial cell death. Low-dose chemotherapy, given at short regular intervals with no prolonged breaks (metronomic chemotherapy), also targets angiogenesis by obliterating proliferating endothelial cells and circulating...

The terminal fate of dendritic cells (DC) remains relatively uncertain. In this study, we tested the hypothesis that DC undergo apoptosis after Ag-specific interaction with T cells. When splenic DC isolated from BALB/c mice were cocultured with HDK-1 T cells (a keyhole limpet hemocyanin (KLH)-specific CD4+ Th1 clone) in the presence of KLH, they showed conspicuous cell death as measured by prop...

The interleukin-3 (IL-3)–dependent murine bone marrow– [PKC] inhibitor), indicating the important role of calciumcalcineurin in this system. On the other hand, the CD95 exderived cell line FDC-P2/185-4 (185-4) undergoes apoptosis when IL-3 is withdrawn from culture medium. Previous repression induced by FcgRIII stimulation was blocked by staurosporine, but not by EGTA or FK506, and phorbol myri...

The interleukin-3 (IL-3)-dependent murine bone marrow-derived cell line FDC-P2/185-4 (185-4) undergoes apoptosis when IL-3 is withdrawn from culture medium. Previous results from our studies indicated that a high concentration of aggregated mouse IgG prevented apoptosis of 185-4 cells through Fc gammaRIII by an autocrine mechanism, producing IL-3. But after 24 hours, 185-4 cells expressed CD95 ...

adverse effects of UV irradiation reinforce the need for novel chemoprevention strategies which involve the use of active compounds from Chinese herbal medicine that might prevent DNA damage or other biological events occurring following UV exposure to the skin. Our previous study demonstrated that silymarin was found to have an anti-apoptotic effect against UV irradiation in human malignant me...

b-thalassemia là một bệnh rối loạn di truyền lặn do đột biến gen HBB nằm trên cánh ngắn nhiễm sắc thể số 11 gây suy giảm tổng hợp chuỗi b-globin. Mỗi năm có khoảng 60.000 – 70.000 trẻ em sinh ra bị toàn thế giới trong đó Việt Nam. Trên 350 đã được công bố. Phát hiện người lành mang vai trò quan trọng tư vấn truyền, chẩn đoán trước và tiền làm tổ nhằm tỉ lệ bệnh. Nghiên cứu thực với mục tiêu “xá...