The objective of this study was to evaluate the usefulness of amplified fragment length polymorphism (AFLP) analyses for selection of the best parents for breeding of hybrid winter triticale. Phenotypic diversity was measured for 8 agronomic traits in 10 parents and 27 F1 hybrids. Genotypic diversity was measured by 91 AFLP markers. Coefficients of correlation of genetic similarity (AFLP-GS) wi...

The PCR-based DNA fingerprinting method called the methylation-sensitive amplified fragment length polymorphism (MS-AFLP) analysis is used for genome-wide scanning of methylation status. In this study, we developed a method of fluorescence-labeled MS-AFLP (FL-MS-AFLP) analysis by applying a fluorescence-labeled primer and fluorescence-detecting electrophoresis apparatus to the existing method o...

one of the best strategies to control bacterial wilt caused by ralstonia solanacearum (smith) is generally based on breeding resistant cultivars. the information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. this study was to identify inducible genes under defense no death (dnd) reaction of tobacco (nicotiana tabac...

Using in silico amplified fragment length polymorphism (AFLP) fingerprints, we explore the relationship between sequence similarity and phylogeny accuracy to test when, in terms of genetic divergence, the quality of AFLP data becomes too low to be informative for a reliable phylogenetic reconstruction. We generated DNA sequences with known phylogenies using balanced and unbalanced trees with re...

Abstract In recent years, tan spot of wheat caused by the fungus Pyrenophora tritici-repentis has become more prevalent in Oklahoma. Experiments were conducted to investigate race structure, disease symptoms and genetic variability P. isolates collected from winter over three decades. Race determination was for 16 based on expression necrosis and/or chlorosis produced differentials. Variability...

Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly in...

Phytoremediation of metal contaminated soils requires high-biomass plants exhibiting tolerance to and accumulation of metal contaminants. However, very little is known about the genes controlling these traits. In order to better understand this, Arabidopsis halleri ssp. halleri (L.) O'Kane and Al-Shehbaz, a naturally selected zinc and cadmium tolerant plant species capable of hyperaccumulating ...