I N T R O D U C T I O N T h e voltage c lamp technique has been used by n u m e r o u s investigators to describe the voltageand t i me -dependen t conductances in cardiac tissue. T h e main conclusion f rom these analyses is that the ionic cur ren ts that under l ie the action potent ial in the hear t are m o r e n u m e r o u s and complex than in the classic squid axon (for reviews, see Trau...

Human neurobalstoma cells, SH-SY5Y, are widely studied and data on their electrophysiological characteristics can be found in literature. We used this cell line to set up the whole cell patch-clamp technique, not yet available in our institute, with the goal to make it suitable both for our future scientific projects and for interdisciplinary studies with other research groups. Some instruments...

Recent measurements have indicated that some of the cardiac cell electrical capacitance is in series with a resistance. The computations of currents in a voltage clamp presented below show that, in this case, there is a danger that capacitive transient currents recorded during voltage clamp experiments may be confused with currents arising through rapid active membrane conductance changes. Seco...

Mechanisms underlying action potential generation in the newt olfactory receptor cell were investigated by using the whole-cell version of the patch-clamp technique. Isolated olfactory cells had a resting membrane potential of -70 +/- 9 mV. Injection of a depolarizing current step triggered action potentials under current clamp condition. The amplitude of the action potential was reduced by low...

The sliding beta clamp subunit of the DNA replication machinery in the bacterium Escherichia coli coordinates multiple functions in the cell beyond genome duplication. In this issue, Parks et al. (2009) find that the beta clamp interacts with the transposition protein TnsE to target the Tn7 transposon to discontinuously replicating DNA at the replication fork.

Introduction. Nephron-sparing surgery is the current gold standard for surgical treatment of localized renal cell carcinoma (RCC). Partial nephrectomy can be performed by off-clamp or on clamp techniques. It especially important to preserve function during partial in comorbid patients. Objective. To evaluate perioperative results robot-assisted with zero ischemia patients RCC. Materials and Met...

Patients carrying the heterozygous A414G mutation in HCN4 gene, which encodes protein, demonstrate moderate to severe bradycardia of heart. Tetramers subunits compose ion channels sinus node that carry hyperpolarization-activated ‘funny’ current (If), also named ‘pacemaker current’. If plays an essential modulating role pacemaker activity. To assess mechanism by results bradycardia, we first pe...