Pancreatic cancer is characterized by excessive desmoplastic reaction and by a hypoxic microenvironment within the solid tumor mass. Chronic pancreatitis is also characterized by fibrosis and hypoxia. Fibroblasts in the area of fibrosis in these pathological settings are now recognized as activated pancreatic stellate cells (PSCs). Recent studies have suggested that a hypoxic environment concom...

Endothelial cells (ECs) form a tight barrier to prevent vascular leakage. Although several studies reported that vascular leakage is increased during hypoxic conditions, other studies describe a protective effect. This raises the question how hypoxia specifically regulates adherens junction integrity and thus endothelial barrier function. Here, we investigated how hypoxia and the hypoxia-mimeti...

An in vitro model of the blood-brain barrier (BBB), consisting of porcine brain-derived microvascular endothelial cells (PBMEC), was used to evaluate the effect of astrocytes in the BBB disruption during hypoxia. Hypoxia-induced hyperpermeability was decreased significantly in a coculture model of astroglia cells, either astrocytes or C6 glioma cells, with PBMEC and, to the same extent, when gl...

Glioblastoma is one of the most aggressive brain tumors with high morbidity and mortality. Hypoxia is often the common characteristic of tumor microenvironment, and hypoxia-inducible factor-1α (HIF-1α) is an essential factor regulating the migratory activity of cancer cells including glioblastoma. Recently, mitochondrial dynamics was found to be involved in the aggression of cancer cells. Howev...

Induced pluripotent stem (iPS)-like cancer cells (iPC) by the introduction of defined transcription factors reduce the prevalence of the malignant phenotype of digestive system cancer cells, but the induction efficiency is low. The role of hypoxia and TP53 deficiency in iPC cell generation remain unclear. Cellular reprogramming was performed by re...

The mouse breast cancer cell lines 4T1, 4T07, and 67NR are highly tumorigenic but vary in metastatic potential: 4T1 widely disseminates, resulting in secondary tumors in the lung, liver, bone, and brain; 4T07 spreads to the lung and liver but is unable to establish metastatic nodules; 67NR is unable to metastasize. The Bcl-2/adenovirus E1B 19 kDa interacting protein-3 (Bnip-3) was recently show...

Activation of acetylcholine receptors (AChRs) exerted cardioprotection against ischemia/reperfusion (I/R) injury. However, the mechanisms underlying its are not fully understood. We investigated effects hypoxia/reoxygenation (H/R), condition that mimics an in vivo I/R, presence and absence AChRs agonists cardiomyoblast H9c2 cells. The cells (104 cells/well) were incubated hypoxic chamber with i...

The molecular mechanism of cell death due to hypoxia has not been elucidated. Our recent observations that overexpression of the anti-apoptotic proto-oncogene bcl-2 and a bcl-2-related gene, bcl-x, prevents hypoxic cell death suggest that hypoxia induces apoptosis. Using electron microscopy and confocal and nonconfocal fluorescence microscopy, we show here that hypoxia does, in fact, induce bot...

Abstract Background Drug resistance and metastasis involving hypoxic tumor environments persistent stem cell populations are detrimental to the survival of patients with non-small lung carcinoma (NSCLC). Tie1 is upregulated in hypoxia believed counteract effectiveness platinum agents by promoting stemness properties cells. We have investigated association HIF-1α cisplatin NSCLC lines. Methods T...