background: bone marrow stromal cells (bmsc) have been successfully employed for movement deficit recovery in spinal cord injury (sci) rat models. one of the unsettled problems in cell transplantation is the relative high proportion of cell death, specifically after neural differentiation. according to our previous studies, p75 receptor, known as the death receptor, is only expressed in bmsc in...

The interrogation of highly diverse repertoires of heterogeneous cell populations on a single cell basis increases the likelihood that a cell with unique characteristics will be identified. We have developed a new single cell analysis system comprising millions of bundled subnanoliter volume bioincubation chambers for the identification and recovery of target specific antibody secreting cells (...

objective: this study was designed to examine the effects of human umbilical cord matrix stemcell (hucmsc) administration in rats for 6 week after traumatic brain injury (tbi). materials and methods: adult male wistar rats (n = 30) were injured with controlled cortical impact device and divided into three groups. the treatment groups (n = 10 each) were injected with 2 × 106 (2 milion) intraveno...

Background/Aim. Thermodynamical and cryobiological parameters responsible for cell damages during cryopreservation (cryoinjuries) have not yet been completely explained. Thus, freezing procedures should be revised, exactly optimized to obtain an enhanced structural and functional recovery of frozen–thawed cells. The aim of this study was to compare microprocessor–controlled (controlled–rate) wi...

BACKGROUND Detection of CTCs is a poor prognostic factor for many cancer types; however, their very low frequency represents an obstacle for their detection. The objective of the current study was to compare the performance of commonly used methods for CTCs isolation. METHODS The evaluated methods using spiking experiments of MCF7, SKBR3 and MDA MB-231 breast cancer cell lines were (i) ficoll...

The data replication problem (DRP) describes the task of distributing copies of data records (that is, database fragments) among a set of servers in a distributed database system. For the application of flexible query answering, several fragments can be overlapping (in terms of tuples in a database table). In this paper, we provide a formulation of the DRP for horizontal fragmentations with ove...

Autologous stem cell transplantation is standard treatment for newly diagnosed younger patients with multiple myeloma and for relapsed or refractory Hodgkin or non-Hodgkin lymphoma. Patient characteristics influencing the yield from stem cell collection and time from transplant to platelet recovery were retrospectively analyzed in 630 consecutive patients, attempting to define adequate amounts ...

Impaired endothelial barrier function results in a persistent increase in endothelial permeability and vascular leakage. Repair of a dysfunctional endothelial barrier requires controlled restoration of adherens junctions, comprising vascular endothelial (VE)-cadherin and associated β-, γ-, α-, and p120-catenins. Little is known about the mechanisms by which recovery of VE-cadherin-mediated cell...

Checkpoint recovery is integral to a successful checkpoint response. Checkpoint pathways monitor progress during cell division so that in the event of an error, the checkpoint is activated to block the cell cycle and activate repair pathways. Intrinsic to this process is that once repair has been achieved, the checkpoint signaling pathway is inactivated and cell cycle progression resumes. We us...

PURPOSE To characterize the effect of repeated brief intraocular pressure (IOP) elevations and the effect of IOP fluctuation on retinal function. METHODS The effects of one, two, and four episodes (70 mm Hg, 15 minutes) are compared by defining the time course of functional recovery after insults. The effect of IOP variation is considered by comparing a constant with a varying insult, keeping...