BACKGROUND The need for effective parameters for selecting the best embryos is paramount when a large number of them are available for transfer. Other studies have reported that transfer of pre-selected embryos, based on cleavage to the 2-cell stage at 25 h and 27 h post-insemination/intracytoplasmic sperm injection (ICSI), increases implantation and pregnancy rates. We investigated whether ext...

My research awarded includes contributions to cryopreservation and sexing of bovine embryos produced in vitro and in vivo, as follows; (1) In vivo-derived morulae and blastocysts were cryopreserved in the presence of 10% glycerol, and the embryos were transferred into recipients after two-step dilution of glycerol in straw, with a practically acceptable pregnancy rate. (2) The survival rate of ...

Embryogenic callus culture of carrot (Daucus carota L.) was induced from the hypocotyl explants in MS medium supplemented with Casein hydrolysate, 2,4-D and BAP for somatic embryogenesis In vitro cell suspension cultures for high multiplication rate. Embryogenic callus was transferred to liquid basal Murashige and Skoog medium The mature somatic embryos were encapsulated in a Sodium alginate mi...

Background: At the outset of the somatic cell nuclear transfer (SCNT) process, the chromatin structure of the somatic cell which governs its state of differentiation undergoes dramatic changes, called reprogramming, and is compelled back to the embryonic stage. However, the overall epigenetic makeup of the resultant cloned embryos has been acknowledged far different from the fertilized embryos....

Protocols for the production and transformation of grapevine embryogenic cultures are described. Embryogenic cultures are initiated from leaves or stamens and pistils and transformed with Agrobacterium containing an enhanced green fluorescent protein/neomycin phosphotransferase II (egfp/nptII) fusion gene. Cultures are transferred to induction medium in the dark for callus formation and prolife...

This report is of a live piglet derived from an embryo produced by intracytoplasmic sperm injection and carried to term. In vivo-matured oocytes were injected with in vitro-capacitated spermatozoa and after 40 h of culture, 2 to 4-cell-stage embryos were transferred to the oviducts of oestrus induced gilts 24 h after the expected ovulation time. Pregnancy of the surrogate mothers was maintained...

introduction: the incidence of chromosomal abnormalities was investigated in untransfered embryos reuslted from in vitro fertilization (ivf) or intracytoplasmic sperm injection (icsi) procedures. materials and methods: a total of 238 embryos of varying morphology between the pronucleated stage and 8-cells were analysed. the cytogenetic method of dyban was used for chromosome preparation. embryo...

BACKGROUND Characteristics routinely used to evaluate embryo quality after thawing include number of blastomeres survived and presence of mitosis resumption after overnight culture. It is unknown to which extent symmetry and fragmentation affect implantation after warming and whether application of stricter criteria either before vitrification or after warming would improve implantation rate (I...

Objective A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somati...