Although tight junctions (TJ) have been extensively studied in simple epithelial cells, it is still unknown whether their organization is coupled to cell differentiation in stratified epithelia. We studied the expression of TJ in RCE1(5T5) cells, an in vitro model which mimics the sequential steps of rabbit corneal epithelial differentiation. RCE1(5T5) cells expressed TJ components which were a...

A monoclonal antibody CC92 (IgM), raised against a fraction of rat liver enriched in Golgi membranes, recognizes a novel Endo H-resistant 74-kD membrane glycoprotein (gp74). The bulk of gp74 is confined to the cis-Golgi network (CGN). Outside the Golgi gp74 is found in tubulovesicular structures and ER foci. In cells incubated at 37 degrees C the majority of gp74 is segregated from the intermed...

This document requests the allocation of an IPv4 /10 address block to be used as Shared Address Space to accommodate the needs of Carrier-Grade NAT (CGN) devices. It is anticipated that Service Providers will use this Shared Address Space to number the interfaces that connect CGN devices to Customer Premises Equipment (CPE). Shared Address Space is distinct from RFC 1918 private address space b...

Coordinated polarization of epithelial cells is a key step during morphogenesis that leads to the formation of an apical lumen. Rab11 and its interacting protein FIP5 are necessary for the targeting of apical endosomes to the midbody and apical membrane initiation site (AMIS) during lumenogenesis. However, the machinery that mediates AMIS establishment and FIP5-endosome targeting remains unknow...

Plexin-B2 deletion leads to aberrant lamination of cerebellar granule neurons (CGNs) and Purkinje cells. Although in the cerebellum is only expressed by proliferating CGN precursors outer external layer (oEGL), its function development still elusive. Here, we used 3D imaging, vivo electroporation live-imaging techniques study novel cerebellum-specific Plxnb2 conditional knockout mice. We show t...

Alpha II-spectrin (alpha-fodrin) is a demonstrated endogenous substrate for caspase-3 in neurons undergoing unscheduled apoptotic death. We have previously identified the caspase cleavage site that yields the distinctive 120 kDa spectrin breakdown product (SBDP120) as (DSLD(1478)*SVEAL). Here, by using a synthetic peptide (NH(2)-SVEALC) mimicking the neo-N-terminal of SBDP120 as antigen, we rep...

Abstract The transcription factor E74-like 3 (ELF3) is inactivated in a range of cancers, including biliary tract cancer (BTC). Here, we investigated the tumor-suppressive role ELF3 bile duct cells by identifying several previously unknown direct target genes that appear to be implicated carcinogenesis. directly repressed ZEB2, key regulator epithelial–mesenchymal transition, and upregulated ex...

This paper describes how the Northern (NL) and Southern (VL) varieties of Dutch are modeled in the joint LIMSIVecsys Research speech-to-text transcription systems for broadcast news (BN) and conversational telephone speech (CTS). Using the Spoken Dutch Corpus resources (CGN), systems were developed and evaluated in the 2008 N-Best benchmark. Modeling techniques that are used in our systems for ...