Combustion of fossil fuels is the main anthropogenic source of mercury. The efficiency of methods for removing mercury from coal syngas or flue gases mainly depends on the chemical forms of mercury. Despite the fact that temperature fractionation is a common approach to defining mercury species in solid samples, the accuracy of known results is still questionable. For developing the method of t...

Although a known regulator of normal hemopoietic cell growth, granulocyte-macrophage colony-stimulating factor (GMCSF) can induce some differentiation in WEHI-3B and M1 leukemic cells [6, 81, ano ther closely rela ted regula tor, macrophage-CSF, is devoid of such activity [l, 71. Moreover, it is becoming clear that the potent differentiation-inducing activity of conditioned media from some cell...

A method for the preparation of HeLa cell plasma membrane ghosts is described. The purity of the plasma membrane fraction was examined by phase contrast and electron microscopy, by chemical analysis, and by assay of marker enzymes. Data on the composition of the plasma membrane fraction are given. It was observed that the distribution pattern of 5'-nucleotidase activity among the subcellular fr...

Historically it has been shown repeatedly that single high doses of radiation do not allow a therapeutic differential between tumor and critical normal tissues but dose fractionation does. The purpose of conventional dose fractionation is to increase dose to the tumor while preserving normal tissue function. Tumors are generally irradiated with 2Gy dose per fraction delivered daily to a more or...

A material suitable for base-pair-specific "affinity chromatography" of double stranded DNA is described. The synthesis of this material involves two successive polymerization reactions yielding solid particles of cross-linked bisacrylamide to which base-pair-specific dyes are covalently attached by spacers of polyacrylamide chains of different length. Materials with immobilized A.T-specific ma...

Heparan sulfate fractions were isolated from three normal human livers and three cancerous human liver tissues, and their polyanionic properties were examined using electrophoresis, sequential partition fractionation, and chemical analyses. More than 60% of total glycosaminoglycans from normal human liver and about 30% from cancerous liver tissue were found to be heparan sulfate from their resi...

Background Many studies underline the great benefits of yeast extracts (YE), used as supplements in animal free culture media, on cell growth and recombinant protein production [1,2]. Nevertheless, their unknown composition and batch-to-batch variability of commercial YE remain constraints for industrial processes [3,4]. Consequently, the identification of bioactive YE molecules is challenging ...

A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-alpha-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the fir...